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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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Universal Hand-held Three-dimensional Optoacoustic Imaging Probe for Deep Tissue Human Angiography and Functional Preclinical Studies in Real Time

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Body fluid diagnostics using activatable optical probes.

Jie Zhan¹, Yanbin Cai², Penghui Cheng³

¹Department of Laboratory Medicine, Guangdong Provincial Key Laboratory of Precision Medical Diagnostics, Guangdong Engineering and Technology Research Center for Rapid Diagnostic Biosensors, Guangdong Provincial Key Laboratory of Single Cell Technology and Application, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China. nfyyzhenglei@smu.edu.cn.

Chemical Society Reviews

|March 14, 2025

Summary

This summary is machine-generated.

Activatable optical probes (AOPs) offer a simpler, more sensitive method for detecting diseases in body fluids. These probes enhance diagnostic accuracy and speed up early disease detection.

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Area of Science:

Biomedical Engineering
Molecular Diagnostics
Optical Sensing

Background:

* In vitro diagnostics are crucial for early disease detection and health monitoring.
* Current optical diagnostic tests often involve complex procedures and lengthy processing times.
* Activatable optical probes (AOPs) offer a promising alternative with enhanced sensitivity and specificity.

Purpose of the Study:

* To review recent advancements in activatable optical probes (AOPs) for body fluid diagnostics.
* To explore the molecular design strategies and diagnostic applications of AOPs.
* To discuss the challenges and future directions of AOPs in clinical diagnostics and precision medicine.

Main Methods:

* Comprehensive literature review of recent research on activatable optical probes.
* Analysis of molecular design principles for AOPs to achieve specific optical readouts.
* Examination of AOP applications in pre-clinical and clinical studies across various diseases.

Main Results:

* AOPs provide higher signal-to-background ratios and improved specificity compared to traditional methods.
* AOPs can simplify diagnostic procedures by eliminating washing steps.
* Demonstrated utility of AOPs in detecting biomarkers for cancer, infectious diseases, and other conditions.

Conclusions:

* Activatable optical probes represent a significant advancement in body fluid diagnostics.
* AOPs have the potential to streamline diagnostic workflows and improve patient outcomes.
* Further research and development are needed to fully realize the clinical potential of AOPs in precision medicine.