Dual-potential ratiometric electrochemiluminescence platform based on PCN and Ti₃CN@luminol for kanamycin detection...

In this paper, a novel dual-potential ratiometric electrochemiluminescence (RECL) sensor was constructed and applied to the ultrasensitive detection of kanamycin (KAN). The RECL sensor was constructed using porous graphite phase carbon nitride (PCN) and luminol, which serve as the luminescent reagents at the cathode and anode, respectively. PCN not only functioned as the luminescent reagent for the cathode but also served as a carrier for the PtAu NPs. Simultaneously, Ti₃CN acted as a carrier for the anode luminescent reagent, luminol, thereby enhancing the ECL signals at anode. Under optimal conditions, the ratio of the anode to cathode ECL signals (I_luminol/I_PCN) showing excellent linear relationship with the logarithm of KAN concentration (lgC_KAN) within the range of 5.0 × 10^-14 to 5.0 × 10^-8 mol/L, with a detection limit as low as 1.67 × 10^-14 mol/L, making it suitable for detecting KAN in milk samples.