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Measurement of C9 concentrations using an immunochemiluminometric assay
Journal of Immunological Methods
|June 12, 1985
Summary
A new assay accurately measures complement component C9 in biological fluids, aiding immune-based disease diagnosis. This stable, non-radioactive method offers superior performance for clinical applications.
Area of Science:
- Clinical Chemistry
- Immunology
- Biochemical Assays
Background:
- Complement component C9 (C9) is crucial in immune-based diseases.
- Accurate measurement of C9 is vital for diagnosis and management.
- Existing immunoassay techniques have limitations.
Purpose of the Study:
- To develop and describe a novel 2-site immunochemiluminometric assay for C9 measurement.
- To evaluate the assay's performance in biological fluids.
- To establish C9 as a biomarker for immune-based diseases.
Main Methods:
- Utilized two monoclonal antibodies, one labeled with chemiluminescent acridinium ester.
- Employed aminoaryl cellulose for antibody immobilization.
- Incubation time of 1 hour with simultaneous reagent addition.
Main Results:
- The assay demonstrated a working range of 10-2500 µg/L with CVs ≤10%.
- Results showed excellent agreement with an established immunoradiometric assay.
- The assay exhibited superior performance compared to other immunochemical methods.
Conclusions:
- The developed assay is a reliable and sensitive tool for C9 quantification.
- It offers a stable, non-radioactive alternative to existing methods.
- This assay can aid in the diagnosis and management of immune-based diseases.