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Updated: May 2, 2026

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Denver Papillae Protocol for Objective Analysis of Fungiform Papillae
Published on: June 8, 2015
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Comparative chromatic study of the interdental gingival papillae
Cristina Gómez Polo1, Javier Montero Martín2, Norberto Quispe López3
1Full Professor, Department of Surgery, School of Medicine, University of Salamanca, Salamanca, Spain.
The Journal of Prosthetic Dentistry
|March 16, 2025
Summary
The color of healthy gingival papillae varies by sex and smoking habits, with women
Area of Science:
- Dental Aesthetics
- Color Science in Dentistry
- Periodontology
Background:
- Limited data exists on interdental papilla color.
- Sociodemographic and behavioral factors' impact on gingival color is unknown.
- Optimal ceramic gingival specimens for matching papilla color require identification.
Purpose of the Study:
- Determine color coordinates of healthy gingival papillae.
- Compare papilla color based on sex, age, hygiene, and smoking.
- Evaluate papilla color against other gingival zones.
- Identify suitable ceramic specimens for subjective color selection.
Main Methods:
- Spectrophotometry used to record color coordinates (L*, a*, b*) of maxillary incisor papillae and attached gingiva in 62 participants.
- Comparison of color coordinates between gingival zones.
- Evaluation of 15 and 133 ceramic gingival specimens against papilla color using acceptability thresholds (AT).
- Statistical analysis using MANOVA and coverage error calculation.
Main Results:
- Interdental papilla color ranges: L* 42.2–57.5, a* 16.8–30.6, b* 11.2–18.1.
- Significant color differences (P<.05) found between all gingival zones.
- Papillae were less yellow and redder than other gingival zones.
- Coverage error for the 15-specimen guide was 2.71.
Conclusions:
- Women's gingival papillae were significantly lighter than men's.
- Smokers exhibited higher b* (yellowness) in papillae compared to non-smokers.
- Age and dental hygiene did not significantly influence papilla color.
- The 15-specimen guide proved effective for subjective shade selection.

