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Updated: May 21, 2025
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Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids
Published on: August 11, 2017
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Microfiber-Templated Porogel Bioinks Enable Tubular Interfaces and Microvascularization Down to the Building Blocks
Yuzhi Guo1, Ziyu Wang1,2, Xuening Zhang1
1Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Biomanufacturing and Engineering Living Systems Innovation International Talents Base (111Base), Department of Mechanical Engineering, Tsinghua University, Beijing, 100084, China.
Small (Weinheim an Der Bergstrasse, Germany)
|March 18, 2025
Summary
A novel microfiber-templated porogel (µFTP) bioink enables 3D bioprinting of vascularized tissues. This approach improves vessel density and in-situ endothelialization, overcoming key challenges in tissue engineering.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Vascularization is critical for biofabricating large-scale tissues.
- Current methods face challenges in vessel density, microvasculature fabrication, and cell distribution.
Purpose of the Study:
- To introduce a new microfiber-templated porogel (µFTP) bioink for engineering vascularized tissue constructs.
- To address limitations in current 3D bioprinting techniques for vascular tissue fabrication.
Main Methods:
- Embedding cell-laden sacrificial microfibers within a porogel bioink for 3D bioprinting.
- Utilizing microfibers to template tubular voids and facilitate in-situ endothelialization.
- Characterizing the rheological properties and printed structure of the µFTP bioink.
Main Results:
- The µFTP bioink supports extrusion-based 3D bioprinting with tunable porosity up to 55%.
- Enhanced endothelial cell growth and spread were observed without post-seeding procedures.
- In vivo studies demonstrated significant promotion of blood vessel and native tissue in-growth.
Conclusions:
- The µFTP bioink approach enables the engineering of tubular bio-interfaces within 3D bioprinted hydrogels.
- This method facilitates in-situ microvasculature endothelialization, offering a versatile tool for vascularized tissue model construction.
