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Enhancement of small intestine function by gastrin
Insights
Gastrin significantly enhances small intestine (SI) development and nutrient absorption in a rat model. This study demonstrates gastrin
Area of Science:
- Gastroenterology
- Developmental Biology
- Endocrinology
Background:
- Infants with small intestine (SI) abnormalities often suffer from malabsorption.
- Gastrin is a potential trophic hormone for SI epithelial cells.
Purpose of the Study:
- To evaluate the effect of gastrin on the function and maturation of developing SI.
- To assess gastrin's impact on nutrient absorption in a rat fetal intestine transplant model.
Main Methods:
- Utilized a rat fetal intestine transplant model with jejunoileal segments.
- Administered gastrin-17 or saline via continuous infusion for 14 days.
- Assessed intestinal maturation via DNA concentration, disaccharidase activity, and nutrient absorption.
Main Results:
- Gastrin significantly increased mucosal DNA concentration (cell mass) (P < 0.01).
- Gastrin administration led to a significant increase in galactose absorption (P < 0.01).
- Gastrin also significantly enhanced glycine absorption (P < 0.05).
Conclusions:
- Exogenous gastrin administration promotes SI maturation and enhances carbohydrate and protein absorption.
- Gastrin shows potential as a therapeutic agent for malabsorptive conditions.
- This study provides the first evidence of exogenous gastrin improving nutrient absorption in a developing SI model.
Abstract:
Frequently, congenital or acquired small intestine (SI) abnormalities in infants lead to inadequate absorption. Gastrin has been suggested as a trophic hormone for SI epithelial cells. We chose to evaluate the effect of gastrin on the function and maturation of developing SI using a rat fetal intestine transplant model. Jejunoileal segments (7-8 cm) obtained from 19 to 20 days gestation fetal rats were implanted subcutaneously in syngeneic adult rats. Two weeks following transplantation the control group (N = 10) was continuously infused with saline and the study group (N = 9) was continuously infused with gastrin-17 (13.5 nM/kg/day) for 14 days. Following the infusion, intestinal maturation and function were evaluated by mucosal DNA concentration, disaccharidase activity, and absorption of [14C]galactose and [14C]glycine. Absorption (microM/cm2 SI) by the control and study groups for galactose was 1.10 +/- 0.18 vs 2.73 +/- 0.25, and for glycine was 1.68 +/- 0.23 vs 2.22 +/- 0.26, respectively. DNA concentration (microgram/mg SI) of the control and study groups was 410 +/- 43 vs 1031 +/- 158, respectively. Lactase and sucrase activity were similar in both groups. Although maltase (microM substrate hydrolyzed/min/g SI) was 13.5 +/- 2.7 for the gastrin group vs 7.9 +/- .9 for the control group, statistical significance was not achieved. Thus, gastrin produced a statistically significant increase in DNA concentration (cell mass) (P less than 0.01). More importantly, to our knowledge, this is the first demonstration that exogenously administered gastrin can increase absorption of carbohydrate (galactose; P less than 0.01) and protein (glycine; P less than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)