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Biological characterization of FPV, Ulster 73, replicative cycle
Microbiologica
|April 1, 1985
Summary
Avian influenza A (FPV) Ulster 73 strain replicates productively in various cell types, unlike the Rostock strain. A viral genetic mechanism likely controls FPV host range and polypeptide synthesis.
Area of Science:
- Virology
- Molecular Biology
- Cell Biology
Background:
- Avian influenza A viruses (FPV) are significant pathogens.
- Understanding FPV replication mechanisms is crucial for disease control.
- Different FPV strains exhibit varying host cell tropism.
Purpose of the Study:
- To investigate the replication pattern of FPV Ulster 73 strain.
- To compare the replication of FPV Ulster 73 with FPV Rostock strain.
- To elucidate the genetic basis of FPV host range determination.
Main Methods:
- Replication studies in chick embryo fibroblasts and various cell lines (LLC-MK2, Hep-2, Vero, KB, Mc Coy).
- Analysis of viral polypeptide synthesis patterns.
- Comparative analysis of FPV Ulster 73 and FPV Rostock replication.
Main Results:
- FPV Ulster 73 demonstrated productive replication in all tested cell lines, including the natural host.
- A characteristic polypeptide synthesis pattern in the natural host suggests strain-specific transcriptional/translational control.
- FPV Ulster 73 exhibited productive replication, whereas FPV Rostock showed abortive replication despite recognizing similar cellular determinants.
Conclusions:
- A viral genetic mechanism controls FPV host range at early and late infection stages.
- This mechanism influences viral-cell molecule interactions, affecting virus-specific polypeptide synthesis.
- FPV Ulster 73 possesses a unique genetic trait enabling broad host cell permissiveness.