Related Experiment Video
Updated: May 21, 2025
Chromogenic In Situ Hybridization as a Tool for HPV-Related Head and Neck Cancer Diagnosis
Published on: June 14, 2019
HPV integration profiling using nanopore sequencing and association with cervical precancerous lesion
Ying Hou1, Shoufeng Ni2, Xin Liu1
1Department of Gynecology, Beijing Youan Hospital, Capital Medical University, Beijing, China.
Objectives:
HPV infection and HPV DNA integration can lead to cervical cancer, but the relationship with lesion severity is unclear. This study aimed to investigate the correlation between HPV integration profile and cervical lesion extent.
Materials And Methods:
Twenty patients representing cervicitis, CIN I, CIN II, and CIN III underwent nanopore sequencing for HPV genotype and integration site analysis. HPV integration profiles were correlated with lesion severity. Gene Ontology (GO) and KEGG analysis were used to identify stage-specific genes and pathways.
Results:
HPV integration rates were 60, 60, 100, and 100% for cervicitis, CIN I, CIN II, and CIN III, respectively, with varying numbers of integrated genes. Each group had specific stage-related genes, with 83 shared genes linked to neuron development and cell-cell processes. CIN II and CIN III displayed more cancer-related pathway enrichment than earlier stages.
Conclusion:
A positive correlation exists between HPV integration frequency and cervical lesion stage. Late-stage lesions showed heightened enrichment in cancer-related pathways through specific HPV-integrated genes.
