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Fish Sperm Assessment Using Software and Cooling Devices

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Sperm Motility Evaluation in Stallion Fresh, Cooled and Frozen Semen Using a Portable Computer-Assisted Sperm

Leonardo F C Brito¹

¹Department of Clinical Studies, New Bolton Center, University of Pennsylvania School of Veterinary Medicine, Kennett, Pennsylvania, USA.

Reproduction in Domestic Animals = Zuchthygiene

|March 20, 2025

Summary

A new portable computer-assisted sperm analysis (CASA) system, the AndroScope, precisely evaluated stallion sperm motility. While showing some kinetic differences, it offers a viable alternative for objective semen analysis in equine breeding.

Keywords:

CASA computer‐assisted sperm analysis fertility semen sperm motility stallion

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Area of Science:

Veterinary Reproduction
Animal Science
Laboratory Diagnostics

Background:

Semen analysis is crucial for stallion breeding management and clinical practice.
Objective evaluation of sperm motility enhances analytical precision.
Computer-assisted sperm analysis (CASA) systems provide objective motility assessment.

Purpose of the Study:

To validate a portable CASA system, the AndroScope, for stallion semen analysis.
To compare the AndroScope's performance against a gold standard CASA system (IVOS II).

Main Methods:

Evaluated fresh/cooled and frozen stallion semen samples using both AndroScope and IVOS II CASA systems.
Compared sperm motility estimates, including percentage of motile sperm and progressively normal sperm.
Assessed sperm kinetic parameters such as velocities, ALH, and BCF.

Main Results:

Strong positive associations (R² = 0.31 to 0.83) were found between AndroScope and IVOS II for all motility estimates.
The AndroScope showed a lower percentage of motile sperm (mean difference: -4.2%) but similar progressively normal sperm percentages compared to IVOS II.
Systemic differences were noted in sperm kinetics (velocities, ALH, BCF), potentially due to the AndroScope's slower video acquisition frame rate (45 Hz vs. 60 Hz).

Conclusions:

The AndroScope system provides precise results for stallion sperm motility parameters.
Its sperm identification, tracking, and kinematic analysis capabilities are comparable to the gold standard.
Users should consider the AndroScope's video acquisition frame rate when interpreting sperm kinetics and classifying motile sperm subpopulations.