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Updated: May 21, 2025
Looking Outwards: Isolation of Cyanobacterial Released Carbohydrate Polymers and Proteins
Published on: May 27, 2019
Structural studies on the O-polysaccharide of Vreelandella alkaliphila strain B521
Elena N Sigida1, Maxim S Kokoulin2, Vlada S Belova3
1Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Centre of the Russian Academy of Sciences (IBPPM RAS), 13 Prospekt Entuziastov, Saratov, 410049, Russia.
Abstract:
Lipopolysaccharide was obtained by hot aqueous-phenol extraction from Vreelandella alkaliphila B521, a moderately halophilic gram-negative bacterium isolated from mud of Lake Botkul, Volgograd Region, Russia. The O-polysaccharide (OPS) was released by mild acid hydrolysis of the lipopolysaccharide and was structurally characterized by chemical analyses and by 1D and 2D NMR spectroscopy. The following structure of the OPS trisaccharide repeating unit was identified: →4)-α-l-GulpNAcA-(1 → 4)-α-l-GulpNAcA-(1 → 6)-α-d-GlcpNAc-(1→.
