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Updated: May 5, 2026
08:57
High-throughput Yeast Plasmid Overexpression Screen
Published on: July 27, 2011
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Advances in CRISPR-enabled genome-wide screens in yeast
Nicholas R Robertson1, Sangcheon Lee2, Aida Tafrishi2
1Bioengineering, University of California, Riverside, Riverside, CA 92521, United States.
FEMS Yeast Research
|March 20, 2025
Summary
Clustered regularly interspaced short palindromic repeats (CRISPR) technology revolutionizes yeast functional genomics. CRISPR-Cas genome-wide screens enable precise gene editing for engineering yeast strains with desirable industrial traits.
Area of Science:
- Synthetic Biology
- Genomics
- Biotechnology
Background:
- CRISPR-Cas systems offer precise gene editing, overcoming limitations of traditional genetic methods.
- Yeast species possess natural traits valuable for industrial biotechnology applications.
- Functional genomics screens are crucial for understanding genotype-phenotype relationships.
Purpose of the Study:
- To review advancements in yeast functional genomics using CRISPR tools.
- To highlight the application of CRISPR-enabled genome-wide screens in metabolic engineering.
- To explore future directions in high-throughput yeast engineering.
Main Methods:
- CRISPR-Cas genome editing for precise gene targeting.
- Genome-wide screens for high-throughput functional genomic investigations.
- Host-first approach to metabolic engineering using nonconventional yeast.
Main Results:
- CRISPR technology enables scalable and specific gene manipulation in yeast.
- CRISPR screens facilitate the engineering of yeast for industrial biotechnology.
- Nonconventional yeast with desirable traits are being developed as production hosts.
Conclusions:
- CRISPR-Cas genome-wide screens are transformative for yeast functional genomics and metabolic engineering.
- The host-first approach leverages natural yeast traits for enhanced production capabilities.
- Future advancements include automation and biosensor-driven screens for accelerated yeast engineering.
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