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Related Concept Videos

CRISPR01:59

CRISPR

48.8K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
48.8K
CRISPR and crRNAs02:53

CRISPR and crRNAs

16.4K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Related Experiment Video

Updated: May 21, 2025

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning
04:17

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

Published on: May 10, 2024

587

Using CRISPR for viral nucleic acid detection.

Maaike S Grimm1, Cameron Myhrvold2

  • 1Department of Molecular Biology, Princeton University, Princeton, NJ, United States.

Methods in Enzymology
|March 22, 2025
PubMed
Summary
This summary is machine-generated.

CRISPR-Cas13 systems offer rapid and sensitive viral nucleic acid detection, improving upon traditional methods. These innovations are crucial for early diagnosis and controlling future viral outbreaks.

Keywords:
CRISPR-Cas based diagnosticsCRISPR-Cas13RNA detectionViral nucleic acids

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Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
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Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

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Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
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Last Updated: May 21, 2025

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning
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Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
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Area of Science:

  • Molecular Biology
  • Virology
  • Biotechnology

Background:

  • Pathogenic viruses pose ongoing threats, necessitating rapid and accurate diagnostic tools.
  • Current viral diagnostics, while sensitive, can have lengthy turnaround times, impeding outbreak control.
  • CRISPR-Cas systems, known for genome editing, show promise for nucleic acid detection.

Purpose of the Study:

  • To review innovations in CRISPR-Cas13-based methods for viral nucleic acid detection.
  • To compare the strengths and weaknesses of various Cas13 detection strategies.
  • To highlight the potential of CRISPR-Cas13 in viral diagnostics.

Main Methods:

  • Review of recent literature on CRISPR-Cas13 applications in viral diagnostics.
  • Analysis of Cas13 enzyme characteristics for nucleic acid detection.
  • Comparison of optimized viral sample collection, amplification, and detection workflows.

Main Results:

  • CRISPR-Cas13 systems demonstrate high sensitivity and specificity for detecting viral nucleic acids.
  • Optimized Cas13 assays offer rapid turnaround times compared to conventional methods.
  • Cas13-based diagnostics show potential for scalability and widespread implementation.

Conclusions:

  • CRISPR-Cas13 technology represents a significant advancement in viral diagnostics.
  • Its rapid and sensitive detection capabilities are vital for managing infectious diseases.
  • Further development of Cas13-based assays will enhance global health security.