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Related Concept Videos

Chemical Shift: Internal References and Solvent Effects01:17

Chemical Shift: Internal References and Solvent Effects

In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...

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Updated: Jun 19, 2026

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
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Interface-edited solid-state NMR to study cell interfaces.

Thomas Kress1, Melinda J Duer2

  • 1Yusuf Hamied Department of Chemistry, University of Cambridge, Cambridge, UK.

Communications Chemistry
|March 23, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed a new solid-state nuclear magnetic resonance (ssNMR) method to analyze the cell glycocalyx in intact cells. This technique provides molecular insights into cell membrane interfaces for biomedical applications.

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Area of Science:

  • Biophysics
  • Structural Biology
  • Cell Biology

Background:

  • Cell membrane interfaces, particularly the glycocalyx, are vital for cellular signaling and molecular interactions.
  • Studying the molecular composition of these interfaces in intact cells is challenging due to limitations in current techniques.

Purpose of the Study:

  • To introduce a novel solid-state nuclear magnetic resonance (ssNMR) tool for characterizing the molecular structure of the cell glycocalyx in intact cells.
  • To enable the study of cell interfaces within their native environment.

Main Methods:

  • Utilized enhanced Goldman-Shen cross-polarization (CP) experiments to generate 13C spectra near cell membranes.
  • Employed interface-edited CP (1D) and PDSD (2D) spectra to obtain detailed spectral information.
  • Probed a region extending up to 10 nm from the cell membrane interface.

Main Results:

  • Successfully demonstrated spectral fingerprints of the mammalian cell glycocalyx using the developed ssNMR method.
  • The technique provides insights into the molecular structure of the cell membrane interface and its surroundings.
  • Preserved membrane composition in a dehydrated, native-like state.

Conclusions:

  • The developed ssNMR tool offers a new approach for studying cell interfaces, including the glycocalyx, in intact cells.
  • This method has significant implications for drug delivery, tissue engineering, and broader biomedical research.
  • Advances the field of structural biology by enabling detailed analysis of cell surface structures.