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CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

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Updated: May 20, 2025

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

CRISPR/Cas System-Based Fluorescent Sensor for Analysis and Detection.

Huiru Niu¹, Lina Zou², Yanan Liu¹

¹Public Health School, Mudanjiang Medical University, Mudanjiang, China.

Critical Reviews in Analytical Chemistry

|March 24, 2025

Summary

This summary is machine-generated.

This review explores Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated protein (CRISPR/Cas) systems and fluorescent sensors. It highlights their combined potential for precise and sensitive molecular analysis and detection applications.

Keywords:

Analysis CRISPR/cas fluorescent sensor trans-cleavage

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Area of Science:

Biotechnology
Molecular Biology
Analytical Chemistry

Background:

Fluorescent sensors enable sensitive detection of analytes via fluorescence principles.
CRISPR/Cas systems offer precise, efficient, and highly sensitive molecular targeting and gene editing capabilities.
Combining CRISPR/Cas with fluorescent sensors presents a powerful approach for advanced diagnostics.

Purpose of the Study:

To review the fundamental mechanisms of CRISPR/Cas systems.
To discuss the characteristics of various Cas proteins.
To summarize the principles and applications of CRISPR/Cas-based fluorescent sensors in analysis and detection.

Main Methods:

Review of existing literature on CRISPR/Cas systems.
Analysis of fluorescent sensor principles.
Synthesis of information on CRISPR/Cas applications in fluorescent sensing.

Main Results:

CRISPR/Cas systems demonstrate high specificity and sensitivity for molecular detection.
Fluorescent sensors provide a versatile platform for signal transduction.
CRISPR/Cas-based fluorescent sensors exhibit excellent performance in various analytical applications.

Conclusions:

CRISPR/Cas systems are highly effective for developing sensitive and specific molecular diagnostic tools.
The integration of CRISPR/Cas technology with fluorescent sensors significantly enhances analytical capabilities.
This review consolidates current knowledge and future directions for CRISPR/Cas-based fluorescent sensors in detection and analysis.