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Isolation and Purification of Plant Extracellular Vesicles from Arabidopsis Leaves Using an Optimized Apoplastic Wash Collection Method
Published on: March 24, 2026
Streptomyces Extracellular Vesicles Promote Plant Defence Responses Through Direct Pathogen Inhibition and Immune
Hang Gao1, Fangfang Jia1, Yueqi Xu2
1College of Biology and Food, Shangqiu Normal University, Shangqiu, China.
Beneficial bacteria-derived extracellular vesicles (EVs) enhance plant immunity. These bacterial EVs inhibit fungal growth and boost plant resistance to pathogens by transferring bioactive molecules.
Area of Science:
- Plant pathology
- Microbiology
- Biochemistry
Background:
- Extracellular vesicles (EVs) mediate molecular transfer between organisms.
- The role of beneficial bacteria-derived EVs in plant defense is largely unknown.
Purpose of the Study:
- Investigate the function of Streptomyces violascens DL157-derived EVs in plant defense.
- Determine if DL157 EVs can enhance plant resistance against pathogens.
Main Methods:
- Isolation of EVs from S. violascens DL157.
- Plant and fungal cell internalization assays.
- Fungal spore germination inhibition assays.
- Plant resistance tests against fungal and bacterial pathogens.
- Salicylic acid and jasmonic acid pathway analysis.
- Multi-omics analyses (metabolomics, proteomics).
Main Results:
- DL157 EVs are internalized by plant and fungal cells.
- DL157 EVs inhibit fungal spore germination.
- DL157 EVs enhance plant resistance to fungal and bacterial pathogens.
- EVs activate both salicylic acid- and jasmonic acid-dependent plant defense pathways.
- Multi-omics data show EVs are rich in bioactive metabolites and proteins.
Conclusions:
- Bacterial EVs play a significant role in plant-microbe interactions.
- DL157 EVs promote plant defense by transferring bioactive molecules.
- EV-mediated communication enhances plant immunity against pathogens.
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