Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Waterpipe smoke inhalation induces lung injury and aortic endothelial dysfunction in mice.

Physiological research·2023
Same author

Ameliorative effect of sesamin in cisplatin-induced nephrotoxicity in rats by suppressing inflammation, oxidative/nitrosative stress, and cellular damage.

Physiological research·2019
Same author

Effect of diesel exhaust particles on renal vascular responses in rats with chronic kidney disease.

Environmental toxicology·2016
Same author

Gum acacia mitigates genetic damage in adenine-induced chronic renal failure in rats.

European journal of clinical investigation·2015
Same author

Influence of treatment with gum acacia on renal vascular responses in a rat model of chronic kidney disease.

European review for medical and pharmacological sciences·2015
Same author

High-mobility group box-1 protein in adenine-induced chronic renal failure and the influence of gum arabic thereon.

Physiological research·2014

Related Experiment Video

Updated: May 20, 2025

Murine Aortic Crush Injury: An Efficient In Vivo Model of Smooth Muscle Cell Proliferation and Endothelial Function
06:14

Murine Aortic Crush Injury: An Efficient In Vivo Model of Smooth Muscle Cell Proliferation and Endothelial Function

Published on: June 11, 2017

8.3K

Direct Effects of Waterpipe Smoke Extract on Aortic Endothelial Cells: An In Vitro Study.

N E Zaaba1, P Yuvarayu, S Beegam

  • 1Department of Physiology, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates. anemmar@uaeu.ac.ae.

Physiological Research
|March 24, 2025
PubMed
Summary

Waterpipe smoking extract (WPSE) damages aortic endothelial cells by increasing oxidative stress and inflammation. This study reveals WPSE triggers cell death and DNA damage, highlighting risks associated with waterpipe smoking.

More Related Videos

Ultrasound Assessment of Flow-Mediated Dilation of the Brachial and Superficial Femoral Arteries in Rats
09:40

Ultrasound Assessment of Flow-Mediated Dilation of the Brachial and Superficial Femoral Arteries in Rats

Published on: November 3, 2016

11.6K
The Aortic Ring Co-culture Assay: A Convenient Tool to Assess the Angiogenic Potential of Mesenchymal Stromal Cells In Vitro
10:04

The Aortic Ring Co-culture Assay: A Convenient Tool to Assess the Angiogenic Potential of Mesenchymal Stromal Cells In Vitro

Published on: September 18, 2017

11.0K

Related Experiment Videos

Last Updated: May 20, 2025

Murine Aortic Crush Injury: An Efficient In Vivo Model of Smooth Muscle Cell Proliferation and Endothelial Function
06:14

Murine Aortic Crush Injury: An Efficient In Vivo Model of Smooth Muscle Cell Proliferation and Endothelial Function

Published on: June 11, 2017

8.3K
Ultrasound Assessment of Flow-Mediated Dilation of the Brachial and Superficial Femoral Arteries in Rats
09:40

Ultrasound Assessment of Flow-Mediated Dilation of the Brachial and Superficial Femoral Arteries in Rats

Published on: November 3, 2016

11.6K
The Aortic Ring Co-culture Assay: A Convenient Tool to Assess the Angiogenic Potential of Mesenchymal Stromal Cells In Vitro
10:04

The Aortic Ring Co-culture Assay: A Convenient Tool to Assess the Angiogenic Potential of Mesenchymal Stromal Cells In Vitro

Published on: September 18, 2017

11.0K

Area of Science:

  • Cardiovascular Biology
  • Cellular Toxicology
  • Environmental Health

Background:

  • Waterpipe smoking (WPS) is linked to adverse health effects, including endothelial dysfunction.
  • Mechanisms involve oxidative stress and inflammation, but direct impact data is scarce.

Purpose of the Study:

  • To investigate the in vitro effects of waterpipe smoke extract (WPSE) on human aortic endothelial cells (TeloHAEC).
  • To elucidate the cellular and molecular mechanisms underlying WPS-induced endothelial damage.

Main Methods:

  • Exposure of TeloHAEC cells to varying concentrations and times of WPSE.
  • Assessed cellular viability, lactate dehydrogenase release, oxidative stress markers (TBARS, SOD, CAT, GSH).
  • Measured inflammatory cytokines (TNF-α), adhesion molecules (E-selectin, ICAM-1), mitochondrial function, DNA damage, apoptosis, and signaling pathways (NF-κB, HIF-1α).

Main Results:

  • WPSE significantly decreased cell viability in a dose- and time-dependent manner.
  • WPSE elevated oxidative stress markers, pro-inflammatory cytokines, and adhesion molecules.
  • WPSE induced mitochondrial dysfunction, DNA damage, apoptosis, and activated NF-κB and HIF-1α pathways.

Conclusions:

  • WPSE directly induces significant cellular damage in aortic endothelial cells.
  • Mechanisms involve oxidative stress, inflammation, DNA damage, mitochondrial dysfunction, and apoptosis.
  • Activation of NF-κB and HIF-1α pathways are key mediators of WPSE-induced endothelial injury.