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Updated: May 20, 2025

Chromatin Isolation by RNA Purification ChIRP
Published on: March 25, 2012
Chromatin Structure Around Long Non-Coding RNA (lncRNA) Genes in Schistosoma mansoni Gonads
Ronaldo C Augusto1, Thomas Quack2, Christoph G Grevelding2
1IHPE, Université de Perpignan Via Domitia, CNRS, IFREMER, Université de Montpellier, 66860 Perpignan, France.
Abstract:
In this study, we employed a total of eight distinct modifications of histone proteins (H3K23ac, H3K27me3, H3K36me3, H3K4me3, H3K9ac, H3K9me3, H4K12ac, and H4K20me1) to discern the various chromatin colors encompassing lncRNA genes in both mature and immature gonads of the human parasite Schistosoma mansoni. Our investigation revealed that these chromatin colors exhibit a tendency to aggregate based on the similarities in their metagene shapes, leading to the formation of less than six distinct clusters. Moreover, these clusters can be further grouped according to their resemblances by shape, which are co-linear with specific regions of the genes, and potentially associated with transcriptional stages.
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