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Related Experiment Video

Updated: May 20, 2025

Intraoperative Visualization of Subretinal Injection and Retinal Detachment in Rats
04:16

Intraoperative Visualization of Subretinal Injection and Retinal Detachment in Rats

Published on: March 7, 2025

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Intraoperative Visualization of Subretinal Injection and Retinal Detachment in Rats.

Alexandre Dentel1, Clémence Bradic2, Ruben Goulet2

  • 1Institut de la Vision, INSERM, CNRS, Sorbonne Université; Department of Ophthalmology, Rothschild Foundation Hospital; alexandre.dentel@inserm.fr.

Journal of Visualized Experiments : Jove
|March 24, 2025
PubMed
Summary
This summary is machine-generated.

This study details a refined subretinal injection technique in rats, improving visualization and control for targeted retinal treatment. This method enhances therapeutic delivery for inherited retinal diseases and research applications.

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Area of Science:

  • Ophthalmology
  • Retinal Surgery
  • Preclinical Research

Background:

  • Subretinal injection delivers therapeutics directly to photoreceptors and retinal pigment epithelium (RPE).
  • Approved therapeutics exist for inherited retinal diseases via subretinal injection.
  • Animal models, especially rodents, present challenges for subretinal injection due to lens size.

Purpose of the Study:

  • To describe a method for subretinal injection in rats with intraoperative visualization and control.
  • To enable precise delivery and retinal detachment management comparable to human procedures.
  • To facilitate preclinical research for retinal diseases and therapies.

Main Methods:

  • Procedure performed under anesthesia with pupil dilation, utilizing an ophthalmic microscope.
  • Subretinal injection achieved through a 30 G scleral channel, creating a retinotomy with the cannula tip.
  • Delivery volumes of 10-25 µL confirmed via fundus photography and optical coherence tomography (OCT).

Main Results:

  • Successful delivery into the subretinal space demonstrated by visible subretinal fluid post-injection.
  • The technique allows for precise intraoperative control of injection site and retinal detachment size.
  • Potential risks include cataract, detachment failure, hemorrhage, and keratitis.

Conclusions:

  • This described method provides enhanced visualization and control for subretinal injections in rats.
  • The technique is valuable for preclinical studies involving targeted retinal therapies and disease modeling.
  • It enables precise induction of retinal detachment for research purposes, mimicking clinical applications.