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Updated: May 20, 2025

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations
Published on: December 7, 2021
Genetic- and culture-based tools for studying Bacteroides fragilis
Matthew K Schnizlein1, Abhishek A Dubey2, Aretha Fiebig1
1Department of Microbiology, Genetics and Immunology, Michigan State University, East Lansing, Michigan, USA.
None:
The relatively limited availability of genetic tools has hampered mechanistic studies of Bacteroides fragilis, an opportunistic anaerobe that constitutes 1%-5% of the gut microbiota in healthy humans. Here we describe novel vectors for B. fragilis gene deletion and expression as well as a semi-defined media for cultivation of B. fragilis str. P207.
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