Related Experiment Video
Updated: Apr 13, 2026

Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides
Published on: July 14, 2015
A cerium phthalocyanine-based covalent organic framework-magnetic core-shell composite as efficient affinity material
Dandan Jiang1, Yangyang Li2, Siyu Wu2
1Inner Mongolia Engineering Research Centre of Lithium-Sulfur Battery Energy Storage, Inner Mongolia Key Laboratory of Carbon Nanomaterials, College of Chemistry and Materials Science, Inner Mongolia Minzu University, Tongliao, 028000, PR China. jdd20091225@163.com.
Abstract:
A novel cerium phthalocyanine-based covalent organic framework (CePc-COF)-magnetic core-shell composite was fabricated by grafting the CePc-COF layer on the surface of Fe3O4. The obtained core-shell composite particle (Fe3O4@CePc-COF) had strong magnetic responsiveness (29.6 emu g-1) and good hydrophilicity (5.0°). The affinity material provided abundant metal ion sites for specific enrichment of phosphopeptides. Fe3O4@CePc-COF had good performance in terms of high selectivity (1: 1: 5000), sensitivity (0.1 fmol), recovery (92.91%), and reusability (10 cycles). The enrichment feasibility of Fe3O4@CePc-COF was first investigated in standard peptides. Furthermore, Fe3O4@CePc-COF can efficiently identify phosphopeptides from extremely complex samples. The work can provide a novel idea for the fabrication of metallophthalocyanine based-COF materials for phosproteome detection in biological samples.
More Related Videos
12:23Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer
Published on: August 2, 2018
10:36Covalent Labeling with Diethylpyrocarbonate for Studying Protein Higher-Order Structure by Mass Spectrometry
Published on: June 15, 2021
Related Concept Videos
Synthesis of Phosphatidylcholine in the ER Membrane
The major components of all eukaryotic cell...
Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Ion-Exchange Chromatography
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)