Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pinching-off of Coated Vesicles01:32

Pinching-off of Coated Vesicles

3.0K
Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
3.0K
Cell Motility through Blebbing01:16

Cell Motility through Blebbing

1.9K
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
1.9K
Rolling Without Slipping01:09

Rolling Without Slipping

3.5K
People have observed the rolling motion without slipping ever since the invention of the wheel. For example, one can look at the interaction between a car's tires and the surface of the road. If the driver presses the accelerator to the floor so that the tires spin without the car moving forward, there must be kinetic friction between the wheels and the road's surface. If the driver slowly presses the accelerator, causing the car to move forward, the tires roll without slipping. It is...
3.5K
Membrane Fluidity01:26

Membrane Fluidity

10.8K
Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is...
10.8K
Intracellular Movement of Viruses and Bacteria01:10

Intracellular Movement of Viruses and Bacteria

2.7K
Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a...
2.7K
The Movement of Organelles and Vesicles01:43

The Movement of Organelles and Vesicles

4.3K
In eukaryotic cells,  cytoskeletal filaments such as actin, microtubules, and intermediate filaments form a mesh-like cytoskeletal network. These filaments serve as tracks for transporting cellular cargo. Specialized motor proteins use the chemical energy stored in adenosine triphosphate (ATP) for this transport. During interphase, microtubules are polarized, with the plus-end towards the cell periphery and the minus-end towards the cell center. Two microtubule-associated motor proteins,...
4.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multimerization interactions between protein-inspired single-chain random heteropolymers.

PloS one·2026
Same author

Quantitative description of protein configuration and viscoelasticity using first-principles hydrodynamics applied to quartz crystal microbalance experiments.

The Journal of chemical physics·2026
Same author

Polymerized Short Sequences as a Template for Protein Folding and Evolution.

Nano letters·2026
Same author

Computational design of functional random heteropolymers through atomistic simulations.

PloS one·2026
Same author

Mechanical performance of hybrid polymer-lipid vesicles with leaflet asymmetry engineered using microfluidics.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Flow-Induced Microdomain Alignment During Block Copolymer Graphoepitaxy.

Advanced materials (Deerfield Beach, Fla.)·2026

Related Experiment Video

Updated: May 20, 2025

Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients
08:15

Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients

Published on: July 16, 2018

7.9K

Rolling vesicles: From confined rotational flows to surface-enabled motion.

Paula Magrinya1, Pablo Palacios-Alonso1,2, Pablo Llombart1

  • 1Department of Theoretical Condensed Matter Physics, Condensed Matter Physics Center, Instituto Nicolás Cabrera, Universidad Autonoma de Madrid, Madrid 28049, Spain.

Proceedings of the National Academy of Sciences of the United States of America
|March 25, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel microfluidic vesicle model to measure friction forces between cell membranes and substrates. This tool quantifies contact friction and lubrication effects, crucial for understanding cell movement and mechanotransduction.

Keywords:
frictionhydrodynamicsrollingvesicles

More Related Videos

Visually Based Characterization of the Incipient Particle Motion in Regular Substrates: From Laminar to Turbulent Conditions
11:51

Visually Based Characterization of the Incipient Particle Motion in Regular Substrates: From Laminar to Turbulent Conditions

Published on: February 22, 2018

8.6K
Spontaneous Formation and Rearrangement of Artificial Lipid Nanotube Networks as a Bottom-Up Model for Endoplasmic Reticulum
07:49

Spontaneous Formation and Rearrangement of Artificial Lipid Nanotube Networks as a Bottom-Up Model for Endoplasmic Reticulum

Published on: January 22, 2019

7.8K

Related Experiment Videos

Last Updated: May 20, 2025

Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients
08:15

Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients

Published on: July 16, 2018

7.9K
Visually Based Characterization of the Incipient Particle Motion in Regular Substrates: From Laminar to Turbulent Conditions
11:51

Visually Based Characterization of the Incipient Particle Motion in Regular Substrates: From Laminar to Turbulent Conditions

Published on: February 22, 2018

8.6K
Spontaneous Formation and Rearrangement of Artificial Lipid Nanotube Networks as a Bottom-Up Model for Endoplasmic Reticulum
07:49

Spontaneous Formation and Rearrangement of Artificial Lipid Nanotube Networks as a Bottom-Up Model for Endoplasmic Reticulum

Published on: January 22, 2019

7.8K

Area of Science:

  • Biophysics
  • Cell Biology
  • Materials Science

Background:

  • Friction forces are critical for cellular locomotion but challenging to measure in vivo due to complex cellular responses.
  • Understanding friction is key to deciphering cell movement and mechanotransduction.

Purpose of the Study:

  • To introduce a synthetic model for precisely measuring friction forces between biomimetic membranes and substrates.
  • To develop a versatile tribological tool for studying friction at the membrane-substrate interface.

Main Methods:

  • Fabrication of microfluidic vesicles with controlled properties encapsulating a single ferromagnetic particle.
  • Magnetic manipulation of the particle to induce controlled vesicle rotation and membrane motion.
  • Analysis of vesicle rolling and slipping behavior across a range of frequencies.

Main Results:

  • The model effectively quantifies contact friction at low frequencies by analyzing molecular contact.
  • At higher frequencies, the model demonstrates lubrication effects leading to vesicle slipping.
  • Vesicle rotation frequency is controllable via magnetic field frequency and vesicle size.

Conclusions:

  • The synthetic vesicle model serves as an effective tribological tool for friction measurement.
  • The model allows for the study of both contact friction and lubrication regimes.
  • Future modifications can incorporate membrane fluidity and ligand-receptor interactions for advanced biomimetic studies.