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Updated: Aug 7, 2026

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Pulse-chase Analysis of N-linked Sugar Chains from Glycoproteins in Mammalian Cells
Published on: April 27, 2010
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PNGase activity and free N-glycans in phloem fluid prepared from Nerium oleander (oleander tree)
Fuki Otaguro1, Yoshinobu Kimura1,2, Megumi Maeda1,3
1Graduate School of Environmental, Life, Natural Science and Technology, Okayama University, Okayama, Japan.
Bioscience, Biotechnology, and Biochemistry
|March 25, 2025
Abstract:
Free N-glycans (FNGs) occur ubiquitously in growing plants. Recently, it was reported that these FNGs interact with auxin. In this study, we investigated whether PNGase activity responsible for producing the FNGs occurs in the extracellular fluid, where auxin is present during its polar transfer. Here, we report the occurrences of PNGase activity and FNGs in the phloem fluid.

