Protein language model-based prediction for plant miRNA encoded peptides

Plant miRNA encoded peptides (miPEPs), which are short peptides derived from small open reading frames within primary miRNAs, play a crucial role in regulating diverse plant traits. Plant miPEPs identification is challenging due to limitations in the available number of known miPEPs for training. Existing prediction methods rely on manually encoded features, including miPEPPred-FRL, to infer plant miPEPs. Recent advances in deep learning modeling of protein sequences provide an opportunity to improve the representation of key features, leveraging large datasets of protein sequences. In this study, we propose an accurate prediction model, called pLM4PEP, which integrates ESM2 peptide embedding with machine learning methods. Our model not only demonstrates precise identification capabilities for plant miPEPs, but also achieves remarkable results across diverse datasets that include other bioactive peptides. The source codes, datasets of pLM4PEP are available at https://github.com/xialab-ahu/pLM4PEP.