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Comparative evaluation of orthodontic mini-implants hygiene protocols on subgingival bacterial load
Amanda Osório Ayres de Freitas1, Ana Paula Vieira Colombo2, Celuta Sales Alviano3
1Federal University of Rio de Janeiro, Dental School, Department of Pediatric Dentistry and Orthodontics (Rio de Janeiro/RJ, Brazil).
Dental Press Journal of Orthodontics
|March 26, 2025
Summary
Mechanical hygiene alone effectively reduces bacteria around orthodontic mini-implants. Adjunctive chemical agents like chlorhexidine did not show superior results, suggesting mechanical cleaning is sufficient for peri-implant health.
Area of Science:
- Oral microbiology
- Orthodontic devices
- Periodontal hygiene
Background:
- Orthodontic mini-implants are crucial for treatment anchorage.
- Maintaining hygiene around mini-implants is essential to prevent bacterial load.
- Various protocols exist to manage peri-implant bacterial contamination.
Purpose of the Study:
- To assess the efficacy of four hygiene protocols in reducing subgingival bacterial load.
- To compare mechanical hygiene with combinations of mechanical hygiene and chemical agents.
Main Methods:
- Thirty-nine participants with 59 mini-implants were randomized into four groups.
- Protocols included mechanical hygiene (M) alone or with chlorhexidine (CHX), triclosan (T), or cetylpyridinium chloride (CP).
- Bacterial load was quantified using real-time PCR on gingival crevicular fluid at baseline and after 21 days.
Main Results:
- All protocols, except mechanical hygiene with cetylpyridinium chloride, significantly reduced bacterial counts from baseline.
- No significant differences in bacterial reduction were found among the evaluated hygiene methods.
- Mechanical hygiene alone demonstrated effectiveness in reducing bacterial load.
Conclusions:
- Mechanical hygiene alone is sufficient to reduce bacterial load and maintain cleanliness of orthodontic mini-implants.
- Adjunctive chemical agents do not offer additional benefits over mechanical cleaning.
- These findings can help prevent overtreatment and guide clinical practice for peri-implant hygiene.

