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Concentration of Metabolites from Low-density Planktonic Communities for Environmental Metabolomics using Nuclear Magnetic Resonance Spectroscopy

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Published on: April 7, 2012

Metagenomic analysis revealed community-level metabolic differences between full-scale EBPR and S2EBPR systems.

Guangyu Li¹, Varun Srinivasan², Nicholas B Tooker³

¹School of Civil and Environmental Engineering, Cornell University, Ithaca, NY, United States; Department of Civil and Environmental Engineering, Northeastern University, Boston, MA, United States.

|March 26, 2025

Summary

Side-Stream Enhanced Biological Phosphorus Removal (S2EBPR) shows distinct microbial communities compared to conventional Enhanced Biological Phosphorus Removal (EBPR). Metagenomics reveals differences in microbial diversity and key phosphorus-accumulating organisms between the two systems.

Keywords:

Comparative full-scale study EBPR Metagenomics Microdiversity PAO

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Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

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Published on: January 7, 2019

Area of Science:

Environmental Microbiology
Wastewater Treatment Technologies
Metagenomics

Background:

Conventional Enhanced Biological Phosphorus Removal (EBPR) faces stability challenges.
Side-Stream Enhanced Biological Phosphorus Removal (S2EBPR) is a promising alternative, but its mechanisms are not fully understood.
Metagenomic analysis can reveal microbial community differences between EBPR and S2EBPR.

Purpose of the Study:

To compare the microbial community structures and functional potentials of EBPR and S2EBPR systems.
To identify key microbial players and metabolic pathways involved in phosphorus removal under different configurations.
To investigate the utility of polyphosphate metabolism genes for differentiating between EBPR and S2EBPR.

Main Methods:

Comparative metagenomic analysis of three EBPR and three S2EBPR activated sludge communities.
Analysis of microbial taxonomy, functional pathways, and polyphosphate metabolism marker genes (ppk and ppx).
Assessment of diversity indices and abundance of specific microbial genera.

Main Results:

S2EBPR communities exhibited greater genus-level diversity variance and a higher overall diversity index than EBPR.
EBPR systems showed higher abundance of Ca. Accumulibacter, while S2EBPR favored fermentative PAOs like Ca. Phosphoribacter and Ca. Promineifilum.
Exopolyphosphatase gene (ppx) phylogeny provided better distinction between EBPR and S2EBPR than polyphosphate kinase gene (ppk).

Conclusions:

S2EBPR and EBPR harbor distinct microbial communities and phosphorus-accumulating organism profiles.
Metagenomics, particularly analysis of specific genes like ppx, can elucidate operational configuration impacts on microbial communities.
Findings offer insights for optimizing wastewater phosphorus removal processes through understanding microbial dynamics.