Related Experiment Video
Updated: May 20, 2025
07:08
Long-term Behavioral and Reproductive Consequences of Embryonic Exposure to Low-dose Toxicants
Published on: March 6, 2018
6.0K
Effects of Lactococcus cremoris PS133 in 5-Hydroxytryptophan-Induced Irritable Bowel Syndrome Model Rats
Fu-Sheng Deng1, Miao-Hui Lin2, Chin-Lin Huang1,3
1Bened Biomedical Co., Ltd., Taipei 115011, Taiwan.
International Journal of Molecular Sciences
|March 27, 2025
Summary
Lactococcus cremoris PS133 reduced irritable bowel syndrome (IBS) symptoms in rats by improving gut barrier integrity and modulating gut microbiota. This probiotic shows promise for managing IBS through enhanced gut health.
Area of Science:
- Gastroenterology
- Microbiology
- Pharmacology
Background:
- Irritable bowel syndrome (IBS) is a functional gastrointestinal disorder characterized by abdominal pain and altered bowel habits.
- Gut microbiota dysbiosis is increasingly recognized as a contributing factor in IBS pathogenesis.
- Probiotics offer a potential therapeutic strategy for restoring gut health and alleviating IBS symptoms.
Purpose of the Study:
- To investigate the effects of Lactococcus cremoris PS133 (PS133) on a rat model of IBS induced by 5-hydroxytryptophan (5-HTP).
- To evaluate PS133's impact on visceral hypersensitivity, intestinal barrier function, neurotransmitter levels, and gut microbiota composition in the IBS model.
Main Methods:
- Sprague Dawley rats were treated with PS133 or saline, followed by 5-HTP administration to induce IBS-like symptoms.
- Visceral hypersensitivity was assessed using colorectal distension tests.
- Intestinal barrier integrity was evaluated by measuring mucin degradation and tight junction protein expression (occludin, zonula occludens-1).
- Neurotransmitter levels (substance P, 5-hydroxyindoleacetic acid) and gut microbiota composition were analyzed.
Main Results:
- PS133 significantly reduced visceral hypersensitivity in the 5-HTP-induced IBS model.
- PS133 protected intestinal mucin from degradation and increased tight junction protein levels, indicating enhanced gut barrier integrity.
- PS133 modulated neurotransmitter levels in the spinal cord and brain and regulated specific gut bacterial groups, including Eubacterium and Lactococcus.
Conclusions:
- Lactococcus cremoris PS133 demonstrates efficacy in ameliorating IBS-like symptoms in a rat model.
- PS133 improves gut barrier function, normalizes neurotransmitter levels, and modulates gut microbiota composition.
- These findings support the potential of PS133 as a therapeutic agent for managing irritable bowel syndrome.
Related Concept Videos
01:27Effects of Chemicals: Overview
1.2K
Drugs, encompassing various chemical compounds from natural sources, lab synthesis, or genetic engineering, elicit different biological responses in living organisms. Some of these responses are desirable or therapeutic, while others are undesirable. The primary goal of administering a drug is to achieve a therapeutic effect, that is, to address a specific disease or health condition. Any concurrent effects outside of this therapeutic outcome are considered undesirable. These undesirable...
1.2K
01:26Toxic Reactions: Overview
920
When toxic substances penetrate the human body, they disseminate to various tissues, undergoing metabolic changes. This process yields reactive metabolites that may covalently bind with specific target molecules, resulting in toxicity.
Toxicity falls into two primary categories: local and systemic.
Local toxicity appears at the exposure site, such as protein denaturation caused by caustic substances.
In contrast, systemic toxicity requires the toxic agent's absorption and distribution,...
Toxicity falls into two primary categories: local and systemic.
Local toxicity appears at the exposure site, such as protein denaturation caused by caustic substances.
In contrast, systemic toxicity requires the toxic agent's absorption and distribution,...
920
01:17Drug Dependence
960
Medications are typically administered to achieve therapeutic effects. Some drugs can modify an individual's mood and perception, frequently resulting in various enjoyable experiences. However, this can result in drug dependency, a condition marked by continuous drug use despite potential negative consequences. Drug dependency primarily falls into two categories: psychological and physical dependence. Psychological dependence occurs when the pleasurable feelings induced by the drug...
960
01:24Effects of feedback
492
Feedback in control systems plays a critical role in shaping various operational parameters, extending beyond simple error reduction to influence stability, bandwidth, gain, impedance, and sensitivity. Understanding these effects requires examining a basic feedback system characterized by defined input, output, error, and feedback signals.
Feedback significantly modifies the gain of a control system. The gain of a system without feedback is altered by a factor of one plus GH, where G represents...
Feedback significantly modifies the gain of a control system. The gain of a system without feedback is altered by a factor of one plus GH, where G represents...
492
01:14Agonism and Antagonism: Quantification
287
When drugs are administered, they can elicit either an agonist or antagonist effect on the body. Agonism occurs when a drug activates a specific receptor, triggering a biological response. On the other hand, antagonism happens when a drug binds to the same receptors but blocks their activation, thereby preventing a biological response.
To quantify these effects, researchers use a dose-response curve, which provides valuable information about the potency and efficacy of a drug. Potency refers to...
To quantify these effects, researchers use a dose-response curve, which provides valuable information about the potency and efficacy of a drug. Potency refers to...
287
01:30Combined Effects of Drugs: Antagonism
8.2K
The combined effects of drugs can result in various interactions, of which an important type is antagonism. Antagonism is a mechanism where one drug inhibits or counteracts the effects of another drug. Antagonism can occur through various means, including receptor binding, allosteric modulation, functional interaction, chemical reactions, and pharmacokinetic processes.
The most common type is receptor antagonism, where one drug acts as an antagonist to block the effects of another drug by...
The most common type is receptor antagonism, where one drug acts as an antagonist to block the effects of another drug by...
8.2K