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Brain cerebrosides: preparative purification using a new chromatographic support--magnammsil
Analytical Biochemistry
|June 1, 1985
Abstract:
A preparative method for isolation and purification of cerebrosides from bovine brain is described. A crude cerebroside fraction is precipitated with ether from a chloroform-methanol extract and purification is achieved by column chromatography on a new column support, "magnammsil." A partial separation of hydroxy and nonhydroxy cerebrosides is achieved. The method is more economical for a large-scale preparation than previously published methods.