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Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...

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An In Vitro Assay to Study Platelet Migration Using RGD-Functionalized Avidin-Biotin Tethers
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Human Platelets in Intravenous Fluids Probed by Raman Tweezers Spectroscopy.

Mithun Nelliat1, Ganesh Mohan2, Shamee Shastry2

  • 1Centre of Excellence for Biophotonics, Manipal Institute of Applied Physics, Manipal Academy of Higher Education, Manipal, Karnataka-576104, India.

Analytical Chemistry
|March 27, 2025
PubMed
Summary

Intravenous fluids (IVs) can activate platelets, potentially leading to harmful effects. This study used Raman tweezers to show IV fluid interactions with platelets, revealing signs of activation like shape change and increased phospholipid expression.

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Area of Science:

  • Biomedical Engineering
  • Hematology
  • Biophysics

Background:

  • Intravenous fluids (IVs) are widely used in medical settings, including pre-transfusion procedures like cell washing.
  • Despite their common use, the impact of IV fluids on platelet function remains under-explored.
  • Platelet activation is critical for hemostasis but excessive activation can be detrimental, particularly in patients with cardiovascular diseases.

Purpose of the Study:

  • To investigate the interaction between live platelets and common intravenous fluids.
  • To determine if IV fluids induce platelet activation using a novel Raman tweezers system.
  • To analyze morphological and biochemical changes in platelets upon exposure to IV fluids.

Main Methods:

  • Utilized a laboratory-assembled Raman Tweezers system to study live platelets.
  • Exposed platelets to various IV fluids: normal saline, hypertonic saline, hypotonic saline, and PlasmaLyte-A.
  • Used platelet additive solution as a control for the experiments.

Main Results:

  • Platelets exposed to IV fluids exhibited increased peak intensities in phospholipid Raman marker bands.
  • These changes indicate heightened surface expression of phospholipids and proteins, signifying platelet activation.
  • Observed morphological alterations included filopodia formation and a shift from discoid to spherical platelet shape.

Conclusions:

  • Commonly used intravenous fluids can induce platelet activation.
  • This activation is evidenced by biochemical and morphological changes in platelets.
  • Further research is needed to understand the clinical implications of IV-induced platelet activation, especially in vulnerable patient populations.