Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Evolutionary Relationships through Genome Comparisons

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...

Sanger Sequencing

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The evolving genetic landscape of telomere biology disorder dyskeratosis congenita.

EMBO molecular medicine·2024

Same author

Protecting against racial bias in DNA databasing.

Nature computational science·2024

Same author

The Y chromosome and its use in forensic DNA analysis.

Emerging topics in life sciences·2021

Same author

Mitochondrial DNA in forensic use.

Emerging topics in life sciences·2021

Same journal

Tissue MicroRNAs in Arrhythmogenic Cardiomyopathy: A Systematic Review of Studies in Human Myocardium and Animal Models with Implications for Post-Mortem Molecular Diagnostics.

Genes·2026

Same journal

Genetic Variants and Dental Caries Susceptibility: An Umbrella Review and Multilevel Meta-Analysis.

Genes·2026

Same journal

Generative AI and Language Models in Human Genetics and Health: From Variant Interpretation to Clinical Decision Support.

Genes·2026

Same journal

Familial White-Sutton Syndrome Caused by a Pathogenic POGZ p.Arg508* Variant: Intrafamilial Variability from Childhood to Adulthood.

Genes·2026

Same journal

Genetic Influence on LDL-Cholesterol Levels: Role of Polygenic Risk Scores and Lp(a) Beyond Monogenic Hypercholesterolemia.

Genes·2026

Same journal

THBS1 as a Key Regulator of Myoblasts: Validation of Its Inhibitory Roles in Skeletal Muscle Development.

Genes·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 5, 2026

Infinium Assay for Large-scale SNP Genotyping Applications

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

Considerations for the Implementation of Massively Parallel Sequencing into Routine Kinship Analysis.

Lucinda Davenport¹, Laurence Devesse¹, Somruetai Satmun¹

¹King's Forensics, Department of Analytical, Environmental and Forensic Sciences, Faculty of Life Sciences and Medicine, King's College London, London SE1 9NH, UK.

|March 28, 2025

Summary

This summary is machine-generated.

Massively parallel sequencing (MPS) offers advanced kinship analysis beyond capillary electrophoresis (CE). This study outlines workflow adaptations for MPS, ensuring accurate genetic relationship testing with sequence-based data.

Keywords:

forensic genetics kinship analysis kinship casework likelihood ratio massively parallel sequencing microhaplotypes next generation sequencing population databases sequence allele nomenclature sequence-based STR analysis

More Related Videos

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Published on: April 4, 2018

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

Related Experiment Videos

Last Updated: May 5, 2026

Infinium Assay for Large-scale SNP Genotyping Applications

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Published on: April 4, 2018

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

Area of Science:

Forensic genetics
Molecular biology
Bioinformatics

Background:

Forensic DNA typing traditionally uses capillary electrophoresis (CE)-based short tandem repeat (STR) analysis for kinship investigations.
CE-based STR analysis has limitations in resolving complex or distant genetic relationships.
Massively parallel sequencing (MPS) is emerging as a powerful alternative for forensic genetic marker analysis, including kinship.

Purpose of the Study:

To adapt existing data analysis workflows for kinship testing to accommodate Massively Parallel Sequencing (MPS) methodologies.
To ensure the full potential of MPS is realized in routine forensic casework while maintaining data integrity.
To provide a framework for laboratories implementing MPS for kinship analysis.

Main Methods:

Utilized empirical data from MPS analysis of forensically relevant STRs and single nucleotide polymorphisms (SNPs).
Incorporated real-world case experience to identify necessary workflow modifications.
Developed adaptations for sequence-based data analysis compatible with existing kinship software.

Main Results:

Identified four key considerations for adapting CE-based workflows to MPS: allele nomenclature, mutational event accounting, population databases, and rare allele frequency handling.
Presented methods for addressing the unique properties of sequence-based data in kinship analysis.
Outlined statistical adjustments for genetic linkage within expanded MPS marker sets.

Conclusions:

This work provides essential guidance for laboratories transitioning to MPS for routine kinship analysis.
The proposed framework addresses data analysis and statistical interpretation challenges specific to MPS.
Implementation of these adaptations will enhance the accuracy and scope of forensic kinship testing.