Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Phases of Wound Repair

Phases of Wound Repair

Following injury, the integrity of the injured tissues must be reestablished. For example, in skin tissue, wound repair involves coordination among resident skin cells, blood mononuclear cells, extracellular matrix, growth factors, and cytokines to complete the healing cascade.
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...

Inflammation

Inflammation

Inflammatory Response II: Inflammatory Exudate and Tissue Repair

Inflammatory Response II: Inflammatory Exudate and Tissue Repair

The immune system's inflammatory response destroys the invading pathogen, permitting the tissue to heal. The changes during the cellular and vascular stages allow exudate formation at the site of inflammation. The inflammatory exudate released from the wound has high protein content and a specific gravity above 1.020.
The typical wound exudate is odorless, transparent, straw-colored, thin, and watery. Exudate, however, can differ depending on the state of wound healing. Likewise, the exudate's...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Engineering the Healing Process: Advanced In Vitro Wound Models and Technologies.

Biomedicines·2026

Same author

The interaction between glucose levels and body mass index on the regulation of the circulating sphingolipidome in humans.

American journal of physiology. Endocrinology and metabolism·2026

Same author

Proteome-Based Biomarkers for Alzheimer's Disease: Old Acquisitions and Innovative Proposals.

International journal of molecular sciences·2025

Same author

Redox Potential (E<sub>0</sub>') of the β-Chain 93Cys of HbS Measured with the Equilibrium Technique in a Heterozygous Sickle Cell Carrier Subject.

Molecules (Basel, Switzerland)·2025

Same author

The Human Amniotic Membrane: A Rediscovered Tool to Improve Wound Healing in Oral Surgery.

International journal of molecular sciences·2025

Same author

Lipid Profile Characterization of Human Micro-Fragmented Adipose Tissue via Untargeted Lipidomics.

Biomolecules·2025

Search research articles

Related Experiment Video

Updated: Jun 16, 2026

Isolation and Characterization of Neutrophil-derived Microparticles for Functional Studies

Isolation and Characterization of Neutrophil-derived Microparticles for Functional Studies

Published on: March 2, 2018

Neutrophil Heterogeneity in Wound Healing.

Filippo Renò¹, Corinna Anais Pagano², Monica Bignotto¹

¹Health Sciences Department (DiSS), San Paolo Hospital, Università di Milano, Via A. di Rudini 8, 20142 Milano, Italy.

|March 28, 2025

Summary

Neutrophil heterogeneity, involving distinct functional states, is crucial for effective wound healing. Understanding these diverse neutrophil roles offers new therapeutic targets for inflammation and healing disorders.

Keywords:

aging inflammation innate immunity neutrophils plasticity skin

More Related Videos

Isolation and Characterization of Neutrophils with Anti-Tumor Properties

Isolation and Characterization of Neutrophils with Anti-Tumor Properties

Published on: June 19, 2015

Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood

Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood

Published on: June 3, 2014

Related Experiment Videos

Last Updated: Jun 16, 2026

Isolation and Characterization of Neutrophil-derived Microparticles for Functional Studies

Isolation and Characterization of Neutrophil-derived Microparticles for Functional Studies

Published on: March 2, 2018

Isolation and Characterization of Neutrophils with Anti-Tumor Properties

Isolation and Characterization of Neutrophils with Anti-Tumor Properties

Published on: June 19, 2015

Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood

Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood

Published on: June 3, 2014

Area of Science:

Immunology
Cell Biology
Wound Healing Research

Background:

Neutrophils are abundant immune cells with underestimated roles.
Neutrophil lifespan and defense mechanisms are more complex than previously thought.
Neutrophil heterogeneity, similar to other immune cells, is increasingly recognized.

Purpose of the Study:

To review the complexity of neutrophil heterogeneity.
To highlight the critical roles of neutrophil subtypes in wound healing.
To explore therapeutic potential related to neutrophil functional states.

Main Methods:

Literature review of neutrophil function in innate immunity and wound healing.
Analysis of factors inducing neutrophil heterogeneity (microenvironment, cytokines, injury type).
Examination of distinct neutrophil subtypes and their functional states.

Main Results:

Neutrophils play roles beyond initial pathogen defense, extending to tissue reconstruction.
Distinct neutrophil subtypes exhibit unique functional states contributing to immunity and healing.
Heterogeneity is influenced by local microenvironment, cytokines, and injury type.

Conclusions:

Neutrophil functional heterogeneity is essential for finely tuned responses to injury and stress.
Understanding neutrophil dynamics in wound healing can identify pathological profiles and therapeutic targets.
Targeting neutrophil heterogeneity may help manage excessive inflammation or impaired healing.