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Updated: Jun 19, 2026

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The Use of an Automated System GreenFeed to Monitor Enteric Methane and Carbon Dioxide Emissions from Ruminant Animals
Published on: September 7, 2015
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Sugarcane Extract (Polygain™) Supplementation Reduces Enteric Methane Emission in Dairy Calves
Richard Osei-Amponsah1,2, Pragna Prathap1,3, Frank R Dunshea1,4
1School of Agriculture, Food and Ecosystem Sciences, The University of Melbourne, Parkville, Melbourne, VIC 3010, Australia.
Animals : an Open Access Journal From MDPI
|March 28, 2025
Summary
Polygain™ (PG), a sugarcane extract, significantly reduced methane emissions in young cattle. This study confirms PG
Area of Science:
- Agricultural Science
- Animal Science
- Environmental Science
Background:
- Livestock farming contributes significantly to global methane (CH4) emissions.
- Polyphenolic compounds show potential for mitigating enteric methane production.
Purpose of the Study:
- To evaluate the efficacy of Polygain™ (PG), a sugarcane-derived polyphenolic extract, in reducing enteric methane emissions from Holstein Friesian calves.
- To assess the impact of PG supplementation on calf growth and development.
Main Methods:
- A completely randomized design experiment involving 24 weaned Holstein Friesian calves over three months.
- Calves were divided into two groups: a control group receiving standard pellets and a treatment group receiving pellets supplemented with 10 g PG/calf/day.
- Enteric methane emissions were measured using a GreenFeed emission monitoring unit (GEM).
Main Results:
- PG supplementation significantly reduced daily methane production in calves by approximately 24.5% (26.66 ± 2.06 g/day) compared to the control group (35.28 ± 1.39 g/day; p < 0.001).
- A significant difference was observed in the CO2/O2 ratio between the treatment and control groups (235 ± 14 vs. 183 ± 9.6; p < 0.001).
- No adverse effects on calf growth and development were observed.
Conclusions:
- Polygain™ supplementation at 10 g/calf/day effectively reduces enteric methane emissions in weaned Holstein Friesian calves.
- PG demonstrates anti-methanogenic potential without negatively impacting animal growth, suggesting its viability as a sustainable livestock feed additive.

