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Correction: Gernhardt et al. Ex Vivo Computed Tomographic Morphometry and Motion of the Native and Fractured Equine Accessory Carpal Bone. <i>Animals</i> 2026, <i>16</i>, 1132.

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Aspirin Eugenol Ester Modulates the Hypothalamus Transcriptome in Broilers Under High Stocking Density.

Xiaodie Zhao1,2, Yi Zhang1,2, Dongying Bai1,2

  • 1Department of Animal Physiology, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China.

Animals : an Open Access Journal From MDPI
|March 28, 2025
PubMed
Summary
This summary is machine-generated.

High-density broiler farming can hinder growth, but aspirin eugenol ester (AEE) supplementation improves performance. This study investigated how AEE affects the broiler hypothalamus transcriptome and growth under high-density conditions.

Keywords:
RNA-seqaspirin eugenol esterbroilerhigh stocking densityhypothalamustranscriptome

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Area of Science:

  • Animal Science
  • Poultry Nutrition
  • Molecular Biology

Background:

  • High-density (HD) stocking in broiler production can lead to competition, negatively impacting growth compared to normal density (ND).
  • Aspirin eugenol ester (AEE) has shown potential to improve growth performance in HD broilers, but the underlying mechanisms, particularly concerning hypothalamus-regulated feeding behavior, remain unclear.

Purpose of the Study:

  • To investigate the effects of dietary AEE supplementation on the hypothalamic transcriptome of broilers under HD conditions.
  • To correlate observed transcriptomic changes with broiler production performance metrics in HD environments.

Main Methods:

  • Four treatment groups were established: Normal Density (ND), High Density (HD), ND with AEE, and HD with AEE, using Arbor Acres broilers.
  • Broiler growth performance (body weight, feed intake, gain, feed conversion) was monitored until day 28.
  • Hypothalamus samples were collected on day 28 for transcriptome analysis.

Main Results:

  • Broilers in the HD group exhibited significantly reduced body weight compared to the ND group (p < 0.01).
  • AEE supplementation significantly improved average daily feed intake, average daily gain, and feed conversion ratio in HD broilers from days 22 to 28 (p < 0.05).
  • Transcriptome analysis revealed 20 commonly enriched signaling pathways between ND vs. HD and HD vs. HD-AEE groups, identifying potential genes involved in CNS development and feed intake regulation.

Conclusions:

  • Dietary AEE supplementation effectively ameliorates the negative effects of high-density housing on broiler growth performance.
  • AEE influences hypothalamic gene expression patterns, offering new insights into the regulation of feeding behavior and growth in broilers under stressful environmental conditions.