Research Progress Fusion Tags for Recombinant Protein Production

Area of Science:

• Biochemistry
• Molecular Biology
• Protein Engineering

Background:

• Recombinant proteins are crucial in various applications but often face challenges in expression, purification, stability, and solubility.
• Structural characteristics of target proteins can impede successful recombinant production.

Purpose of the Study:

• To review commonly used fusion tags in recombinant protein production.
• To summarize the functions of these fusion tags.
• To highlight novel fusion tags and their potential applications.

Main Methods:

• Review of scientific literature on recombinant protein production techniques.
• Analysis of fusion tag strategies for improving protein characteristics.
• Summary of existing and emerging fusion tag technologies.

Main Results:

• Fusion tags, when appended to recombinant proteins, can significantly enhance solubility, stability, and yield.
• Tags can range from short peptides to entire protein domains, aiding in purification and conferring new properties.
• Both established and novel fusion tags offer diverse solutions to protein production challenges.

Conclusions:

• Fusion tag technology is essential for overcoming limitations in recombinant protein expression and purification.
• Strategic use of fusion tags can optimize the production of functional recombinant proteins for diverse applications.
• Continued development of novel fusion tags promises further advancements in protein engineering.