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Updated: Jul 13, 2026
11:13
Using Mouse Oocytes to Assess Human Gene Function During Meiosis I
Published on: April 10, 2018
Localization and function of APC15 during mouse oocyte meiotic progression
Shi-Bin Chao1,2, Ren-Ren Zhang3,4, Qing-Yuan Sun5,6,7
1Reproductive Medicine Center, Yancheng Maternal and Child Health Care Hospital Affiliated to Yangzhou University, Yancheng, Jiangsu, China.
Journal of Molecular Histology
|March 28, 2025
Summary
APC15 is crucial for mouse oocyte meiosis, regulating the Spindle Assembly Checkpoint (SAC) and metaphase-anaphase transition. Its disruption causes meiotic arrest, impacting chromosomal stability.
Area of Science:
- Cell Biology
- Molecular Biology
- Reproductive Biology
Background:
- The Anaphase-Promoting Complex/Cyclosome (APC/C) is vital for cell cycle control.
- APC15 is an essential APC/C subunit, but its meiotic function is unclear.
- Understanding APC15 in oocytes is key for reproductive health.
Purpose of the Study:
- Investigate APC15 expression, localization, and function in mouse oocyte meiosis.
- Determine APC15's role in meiotic progression and the Spindle Assembly Checkpoint (SAC).
- Elucidate APC15's contribution to chromosomal stability during oocyte maturation.
Main Methods:
- Immunofluorescence and confocal microscopy for APC15 localization.
- siRNA-mediated knockdown of APC15 in mouse oocytes.
- Analysis of spindle organization, BUB3 kinetochore removal, and meiotic progression.
Main Results:
- APC15 shows dynamic localization during oocyte meiosis.
- APC15 knockdown causes metaphase I arrest and impairs BUB3 removal.
- Spindle organization remains unaffected by APC15 knockdown.
Conclusions:
- APC15 is essential for SAC inactivation and metaphase-anaphase transition in oocytes.
- APC15 plays a critical role in regulating meiotic progression.
- APC15 is important for maintaining chromosomal stability in maturing oocytes.
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