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Primer-Free Aptamer Selection Using A Random DNA Library
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Enabling Sensitive Quantification of Exosomes Combining Aptamer-Based Rolling Circle Amplification and Silver

Qing Dong1,2, Xu Sun1, Yuling Wang3

  • 1State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun, Jilin 130022, P. R. China.

Analytical Chemistry
|March 28, 2025
PubMed
Summary

This study introduces a novel, cost-effective method for detecting exosomes, crucial biomarkers for disease. The technique offers high sensitivity and specificity for potential clinical applications in liquid biopsy.

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Area of Science:

  • Biotechnology
  • Nanotechnology
  • Biomarker Discovery

Background:

  • Exosomes are vital biomarkers in body fluids for disease diagnosis and prognosis.
  • Current exosome detection methods lack sensitivity, specificity, and cost-effectiveness for clinical use.

Purpose of the Study:

  • To develop a fast, accurate, and cost-effective method for exosome detection.
  • To enable sensitive and specific exosome quantification for clinical applications.

Main Methods:

  • Utilized CD63 aptamer-functionalized magnetic beads for exosome capture.
  • Employed rolling circle amplification (RCA) for signal amplification upon exosome binding.
  • Developed a silver nanoparticle (AgNP) aggregation assay for visual or spectroscopic detection.

Main Results:

  • Achieved a limit of detection of 4.0 × 10^4 particles/mL visually and 800 particles/mL via UV-vis spectroscopy.
  • Demonstrated successful exosome detection in human serum samples.
  • The method is rapid, accurate, and cost-effective.

Conclusions:

  • The developed method provides a sensitive, specific, and cost-effective approach for exosome detection.
  • This technique holds significant potential for clinical applications, particularly in liquid biopsy for disease diagnosis and prognosis.