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Updated: Jun 1, 2026
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A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting
Published on: March 9, 2017
Well-Established Long-Lasting Chemiluminescence Platform Based on Deep Eutectic Solvents.
Zhangpeng Xu1,2, Sipeng Tian1,3, Tongtong Zhai1,3
1Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China.
Analytical Chemistry
|May 30, 2026
Summary
This study introduces a novel deep eutectic solvent (DES) platform for sustained chemiluminescence (CL). This system achieves long-lasting CL for up to 72 hours by controlling reactant diffusion, enabling sensitive H2O2 and ALP detection.
Area of Science:
- Analytical Chemistry
- Materials Science
- Chemical Engineering
Background:
- Long-lasting chemiluminescence (CL) offers advantages over flash-type systems but often requires complex microreactors to control diffusion.
- Existing methods for sustained CL face challenges in managing mass transport for prolonged emission.
- Deep eutectic solvents (DESs) present a potential alternative for microreactor fabrication due to their tunable properties.
Purpose of the Study:
- To develop a facile "all-in-one" mixing platform for achieving long-lasting chemiluminescence (CL) emission.
- To utilize deep eutectic solvents (DESs) as integrated microreactors for controlled reactant diffusion.
- To establish a sensitive detection method for hydrogen peroxide (H2O2) and alkaline phosphatase (ALP) using the developed CL system.
Main Methods:
- Systematic screening of DESs identified choline chloride-urea (ChCl-urea) as optimal due to its viscosity and melting point.
- Cobalt(II) ions (Co2+) were pre-incorporated into the DES (Co@DES) via coordination bonds for catalytic activity and dispersibility.
- The Co@DES acted as a controlled-release reservoir, delivering Co2+ to an aqueous luminol phase, while enriching luminol at the interface.
Main Results:
- The developed DES platform sustained chemiluminescence (CL) for up to 72 hours, a significant improvement over conventional aqueous systems (decay within 5 minutes).
- Retarded diffusion of Co2+ from the DES facilitated continuous generation of reactive oxygen species, enabling sustained CL emission.
- Sensitive detection of H2O2 and alkaline phosphatase (ALP) was achieved based on integrated CL intensity, duration, and catalytic characteristics.
Conclusions:
- Deep eutectic solvents (DESs) provide a generalizable and effective strategy for modulating diffusion and achieving long-lasting chemiluminescence (CL).
- The "all-in-one" DES mixing platform offers a simplified approach compared to complex microreactors for sustained CL applications.
- The developed system demonstrates significant potential for sensitive biosensing and immunoassays, particularly for H2O2 and ALP detection.
