Protocol for spatial metabolomics and isotope tracing in the mouse brain

Area of Science:

• Neuroscience
• Analytical Chemistry
• Biochemistry

Background:

• Mass spectrometry imaging (MSI) offers high-resolution spatial chemical mapping.
• Dynamic analysis with tracers in MSI presents significant challenges.
• Understanding brain metabolism and tracer dynamics is crucial.

Purpose of the Study:

• To develop and present a protocol for spatial metabolomics and isotope tracing in the mouse brain.
• To overcome challenges in dynamic MSI analysis for brain research.
• To enable region-specific tracing of metabolites and tracers.

Main Methods:

• Detailed protocol for tracer administration and mouse brain tissue collection.
• Cryosectioning and matrix application procedures.
• Ion identification and spatial data analysis techniques.

Main Results:

• The protocol yields high-quality spatial metabolomics data.
• Successful implementation of isotope tracing in the mouse brain.
• Demonstrated suitability for region-specific analysis.

Conclusions:

• The presented protocol enhances spatial metabolomics capabilities in the brain.
• It is well-suited for dynamic analysis using isotope tracers.
• Facilitates detailed investigation of brain chemistry and metabolism.