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Adipocyte enhancer-binding protein 1 (AEBP1) knockdown suppressed hepatocellular carcinoma (HCC) cell proliferation and increased apoptosis by inhibiting the PI3K/AKT pathway. AEBP1 may be a therapeutic target for HCC, though its role in invasion and migration is limited.

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Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Adipocyte enhancer-binding protein 1 (AEBP1) is implicated in collagen fibrosis, extracellular matrix regulation, and oncogenic pathways.
  • The specific role of AEBP1 in hepatocellular carcinoma (HCC) remains uncharacterized.

Purpose of the Study:

  • To investigate the impact of AEBP1 knockdown on HCC cell proliferation, apoptosis, migration, and invasion.
  • To elucidate the effect of AEBP1 on the PI3K/AKT signaling pathway in HCC.

Main Methods:

  • Utilized MHCC-97H and Huh7 HCC cell lines.
  • Employed AEBP1 siRNA (siAEBP1) for gene knockdown.
  • Assessed cell proliferation (CCK-8, EdU), apoptosis (TUNEL), invasion (Transwell), migration (scratch assay), and PI3K/AKT pathway activation (Western blot).

Main Results:

  • siAEBP1 transfection significantly reduced AEBP1 expression, inhibited cell proliferation, and induced apoptosis in both cell lines.
  • AEBP1 knockdown downregulated p-PI3K/PI3K and p-AKT/AKT expressions.
  • While proliferation and apoptosis were affected, the impact on invasion and migration varied between cell lines.
  • Rescue experiments confirmed the involvement of the PI3K/AKT pathway.

Conclusions:

  • AEBP1 knockdown inhibits HCC cell proliferation and induces apoptosis, primarily through the PI3K/AKT pathway.
  • AEBP1 demonstrates potential as a therapeutic target for HCC.
  • The influence of AEBP1 on HCC invasion and migration appears context-dependent and potentially limited.