One-step cascade method via glucose oxidase-copper ion complex for detecting glucose using a portable device

Area of Science:

• Analytical Chemistry
• Biochemistry
• Materials Science

Background:

• Accurate glucose detection in honey is crucial for quality control.
• Existing methods often face challenges with pH compatibility and complex procedures.
• There is a need for rapid, streamlined analytical techniques for honey analysis.

Purpose of the Study:

• To develop a one-step cascade fluorescence method for sensitive glucose detection in honey.
• To utilize glucose oxidase-copper ion complexes (GOx@Cu2+) with dual enzymatic activities.
• To simplify the detection process, reduce analysis time, and enable on-site measurements.

Main Methods:

• Development of glucose oxidase-copper ion complexes (GOx@Cu2+) with glucose oxidase and peroxidase-like activities.
• Catalysis of a cascade reaction involving glucose and o-phenylenediamine (OPD) to form fluorescent 2,3-diaminophenazine (oxOPD).
• Validation using honey samples, capillary electrophoresis, and a portable detection device.

Main Results:

• The method achieved high recoveries (96.4%-106%) and low relative standard deviations (<1.9%) in honey samples.
• Demonstrated selectivity and accuracy through capillary electrophoresis analysis.
• Successful on-site glucose detection using a self-designed portable device.

Conclusions:

• The developed one-step cascade fluorescence method offers a rapid, efficient, and simplified approach for glucose detection in honey.
• The GOx@Cu2+ complexes effectively overcome pH mismatch issues and streamline the analytical workflow.
• The portable device facilitates on-site analysis, enhancing the practicality of the method for honey quality assessment.