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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Gastrulation01:56

Gastrulation

Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata will form...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

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Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
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Alternative splicing dynamics during gastrulation in mouse embryo.

Wei Wang1, Yu Zhang1, Yuanyuan Zhai1

  • 1Inner Mongolia Key Laboratory of Life Health and Bioinformatics, School of Life Science and Technology, Inner Mongolia University of Science and Technology, Baotou, China.

Scientific Reports
|March 30, 2025
PubMed
Summary
This summary is machine-generated.

Alternative splicing (AS) dynamics and splicing factor (SF) expression are crucial during mouse embryo gastrulation. This study reveals key AS events and regulatory mechanisms in the developing germ layers.

Keywords:
Alternative splicingEmbryonic developmentEpigenetic regulationMouse gastrulationSplicing factors

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Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Epigenetics

Background:

  • Alternative splicing (AS) is vital for development, differentiation, and cancer.
  • Mechanisms of splicing regulation during mouse gastrulation are not fully understood.
  • Gastrulation establishes the three primary germ layers in mammalian embryos.

Purpose of the Study:

  • To investigate the dynamics of alternative splicing during mouse gastrulation (E6.5-E7.5).
  • To reveal the regulatory mechanisms of AS across primary germ layers.
  • To characterize the expression patterns and functions of splicing factors (SFs) during gastrulation.

Main Methods:

  • Analysis of spatial-temporal transcriptome and epigenome data.
  • Detection and characterization of AS events and differential alternative splicing events (DASEs).
  • Profiling of splicing factor (SF) expression and associated epigenetic signals.

Main Results:

  • AS and DASEs show dynamic changes, significantly increasing in late gastrulation.
  • SFs exhibit stage-specific expression, with elevated levels in middle and late gastrulation.
  • Epigenetic signals linked to SFs and AS sites are enriched and dynamically change during gastrulation.

Conclusions:

  • This study provides a systematic analysis of AS during mouse gastrulation.
  • Identifies primary germ layer-specific AS events and characterizes SF expression and epigenetic regulation.
  • Enhances understanding of germ layer formation mechanisms, focusing on pre-mRNA AS.