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Updated: May 17, 2025

PIP-on-a-chip: A Label-free Study of Protein-phosphoinositide Interactions
Published on: July 27, 2017
The potential impact of lipopolysaccharides (LPS) interactions on light-activated ion channels and pumps
Youngwoo Kim1,2, Jiwoo Kim1,3, Victoria Spedding4
1Biology, University of Kentucky, Lexington, Kentucky, United States.
Abstract:
Optogenetics, the expression of light-activated ion channels and pumps, has the potential in therapeutic treatments for various pathologies. Thus, it is of interest to investigate pharmacological agents, immunological responses, and disease states like bacterial septicemia to learn if light-activated ion channels and pumps are compromised. Bacterial septicemia may impede the function of light-activated channels due to direct actions of lipopolysaccharides. This study explored the effects of activating light-sensitive channels (channelrhodopsin and halorhodopsin) before, during, and after exposure of lipopolysaccharides. Lipopolysaccharide itself did not directly impede the action of LPS but varied because of the effect on ion gradients by changes in membrane potential by lipopolysaccharides.
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