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Related Experiment Video

Updated: Jun 28, 2026

A Protocol for Decellularizing Mouse Cochleae for Inner Ear Tissue Engineering

A Protocol for Decellularizing Mouse Cochleae for Inner Ear Tissue Engineering

Published on: January 1, 2018

An update on technical method of cartilage decellularization: A physical-based protocol.

Hengameh Dortaj^1,2, Ahmad Vaez¹, Ashraf Hassanpour-Dehnavie³

¹Department of Tissue Engineering and Applied Cell Sciences, School of Advance Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.

Bioimpacts : BI

|March 31, 2025

Summary

This study developed a novel physical decellularization method for cartilage extracellular matrix (ECM) scaffolds. The optimized protocol effectively removes cells while preserving ECM structure, showing promise for cartilage tissue engineering without toxic chemicals.

Keywords:

Cartilage Extracellular matrix Physical decellularization Tissue engineering

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Last Updated: Jun 28, 2026

A Protocol for Decellularizing Mouse Cochleae for Inner Ear Tissue Engineering

A Protocol for Decellularizing Mouse Cochleae for Inner Ear Tissue Engineering

Published on: January 1, 2018

Fabrication of Decellularized Cartilage-derived Matrix Scaffolds

Fabrication of Decellularized Cartilage-derived Matrix Scaffolds

Published on: January 7, 2019

Synthesis of Decellularized Cartilage Extracellular Matrix Hydrogels

Synthesis of Decellularized Cartilage Extracellular Matrix Hydrogels

Published on: July 21, 2023

Area of Science:

Biomaterials Science
Tissue Engineering
Orthopedic Surgery

Background:

Cartilage defects lack effective treatments, driving research in tissue engineering.
Decellularized extracellular matrix (ECM) is a promising scaffold for cell growth.
Detergent-based decellularization can damage ECM, hindering recellularization.

Purpose of the Study:

To optimize cartilage decellularization using physical methods.
To avoid ionic detergents in the decellularization process.
To create a biocompatible and bioactive cartilage scaffold.

Main Methods:

Bovine tracheal cartilage was decellularized using freeze-thaw cycles and ultrasound.
Tissues were treated with 0.25% trypsin.
Histological, cytocompatibility, and in-vivo assessments were performed.

Main Results:

Histology confirmed efficient cell removal and ECM preservation.
Fibroblast cell culture showed successful proliferation and migration.
In-vivo studies revealed reduced immune response and leukocyte infiltration.

Conclusions:

Physical decellularization efficiently removes cells while preserving ECM.
The resulting scaffold is suitable for cartilage reconstruction.
This method avoids toxic chemicals, enhancing scaffold safety.