Injectable Hydrogel Loaded with Plasma-Rich Platelets Repairing Endometrial Injury and Remodeling Reproductive...

Area of Science:

• Biomaterials Science
• Regenerative Medicine
• Reproductive Biology

Background:

• Uterine damage presents a significant challenge to fertility and societal development.
• Current treatments for uterine injury are limited by the complex uterine environment.
• Platelet-rich plasma (PRP) shows potential for uterine repair but suffers from rapid factor release.

Purpose of the Study:

• To synthesize a novel biodegradable hydrogel for sustained delivery of PRP to repair uterine damage.
• To evaluate the efficacy of the PRP-loaded hydrogel (HOHP) in promoting uterine tissue regeneration and restoring fertility.

Main Methods:

• Synthesis of a biodegradable, injectable, self-healing hydrogel from natural polysaccharides via dynamic Schiff base chemistry.
• Loading of PRP into the hydrogel's porous structure for sustained release.
• In vitro assessment of cell proliferation, migration, and vascular regeneration.
• In vivo evaluation of the hydrogel's efficacy in a uterine endometrial injury model.

Main Results:

• The synthesized hydrogel exhibited excellent tissue adhesion, self-healing, and injectability.
• In vitro studies showed HOHP significantly enhanced cell proliferation, migration, and vascular regeneration by upregulating VEGFA and activating the PI3K/AKT pathway.
• In vivo experiments demonstrated HOHP effectively restored endometrial thickness, gland number, reduced collagen deposition, and promoted angiogenesis, leading to fertility restoration.

Conclusions:

• The developed polysaccharide-based hydrogel provides a promising platform for sustained PRP delivery for uterine repair.
• HOHP demonstrates significant potential for clinical application in managing uterine injury and improving fertility outcomes.