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Updated: May 16, 2025
In vivo Imaging of Optic Nerve Fiber Integrity by Contrast-Enhanced MRI in Mice
Published on: July 22, 2014
T1-weighted fMRI in mouse visual cortex using an Iron Oxide Nanoparticle contrast agent and Ultrashort Echo Time
Purpose:
This study aims to investigate the feasibility of using T1-weighted fMRI with an iron oxide nanoparticle contrast agent and Ultrashort Echo Time (UTE) imaging at 9.4T to measure functional hyperaemia in the mouse visual cortex. The goal is to capture positive signal changes in both the parenchyma and pial surface, and to test whether surface vessels respond during neuronal activation.
Methods:
The study involved scanning of nine mice after administration of iron oxide-based superparamagnetic contrast agent (Molday ION) via the tail vein. Two functional imaging experiments were conducted: one to investigate the effect of echo time on the functional response, and another to characterize the impact of higher resolution on UTE functional contrast. Regions of interest (ROIs) were defined in the parenchyma and pial surface of the visual cortex.
Results:
The administration of the contrast agent produced a bright-blood signal in the vasculature in structural MRI when using a UTE acquisition. Positive signal changes were observed at the shortest echo time (0.164 ms) in both the parenchyma (0.2% +/- 0.08) and pial surface (0.2% +/- 0.1 %), providing evidence that UTE fMRI experiments can detect changes in both pial and parenchymal vessels. Measurements using longer echo times (≥1 ms) showed negative signal changes. Higher spatial resolution resulted in increased percent signal change at the pial surface, suggesting less partial volume effects and better delineation of surface vessels.
Conclusion:
The findings demonstrate that T1-weighted fMRI with UTE imaging and iron oxide nanoparticles captures positive signal changes across all vascular compartments, providing additional insights into the involvement of surface vessels during functional hyperemia.
