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Related Experiment Video

Updated: May 16, 2025

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
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Microfluidic Nano-Plasmonic Imaging Platform for Purification- and Label-Free Single Small Extracellular Vesicle

Omid Mohsen Daraei, Avinash Kumar Singh, Saswat Mohapatra

    Biorxiv : the Preprint Server for Biology
    |April 1, 2025
    PubMed
    Summary

    This study introduces a novel microfluidic chip for label-free, single small extracellular vesicle (sEV) counting in plasma. This purification-free method offers sensitive and efficient detection of circulating sEVs for disease diagnosis.

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    Area of Science:

    • Biomedical Engineering
    • Nanotechnology
    • Clinical Diagnostics

    Background:

    • Circulating small extracellular vesicles (sEVs) are promising non-invasive biomarkers for disease diagnosis.
    • Quantitative detection of sEVs in complex blood plasma is challenging, often requiring extensive sample preparation.
    • Current methods for sEV analysis can be time-consuming and prone to manual errors.

    Purpose of the Study:

    • To develop a purification-free method for sensitive and efficient single sEV counting in plasma.
    • To demonstrate the utility of a microfluidic chip integrated with Plasmonic nano-aperture label-free imaging (PANORAMA) for sEV detection.
    • To establish a rapid and automated platform for analyzing circulating sEVs.

    Main Methods:

    • A microfluidic chip functionalized with CD63, CD9, and CD81 antibodies on arrayed gold nanodisks on invisible substrate (AGNIS) was developed for selective sEV capture.
    • The chip was integrated with PANORAMA for label-free, single sEV counting.
    • An automated platform allowed precise control over flow parameters and analysis of 20 µL plasma samples within 60 minutes.

    Main Results:

    • The developed platform enabled label-free, single sEV counting directly from plasma without purification.
    • The system demonstrated high sensitivity and efficiency in detecting circulating sEVs.
    • The automated microfluidic approach minimized manual errors and standardized the analysis process.

    Conclusions:

    • This purification-free microfluidic chip integrated with PANORAMA presents a sensitive and efficient tool for detecting circulating sEVs.
    • The platform holds significant potential for advancing non-invasive disease diagnosis through sEV biomarker analysis.
    • The automated, rapid analysis of small plasma volumes offers a practical solution for clinical applications.