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Updated: May 19, 2026

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

Isolation and Purification-Free Digital Single-Small Extracellular Vesicle Biosensing with Scalable Plasmonic Arrays.

Mohammad Sadman Mallick¹, Saswat Mohapatra¹, Abhay Kotnala¹

¹Department of Electrical and Computer Engineering, University of Houston, 4800 MLK Blvd., Houston, Texas 77204, United States of America.

Biorxiv : the Preprint Server for Biology

|May 18, 2026

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A new lithography method (NSSL) enables uniform, large-area plasmonic biosensors for digital analysis of extracellular vesicles. This scalable platform allows sensitive detection and molecular profiling for liquid biopsy applications.

Area of Science:

Nanotechnology and Nanoscience
Biomedical Engineering
Analytical Chemistry

Background:

Plasmonic biosensing enables label-free analysis of single nanoparticles like extracellular vesicles (sEVs).
Previous methods like PANORAMA offer isolation-free sEV analysis but face fabrication uniformity challenges with nanosphere lithography (NSL).

Purpose of the Study:

To develop a scalable and uniform fabrication method for plasmonic biosensors.
To enhance the capabilities of PANORAMA for high-throughput, digital single-sEV analysis and molecular profiling.

Main Methods:

Introduced nanosphere settling lithography (NSSL) as an alternative to NSL for fabricating arrayed gold nanodisks on invisible substrates (AGNIS).
Utilized PANORAMA for digital counting, sizing, and fluorescence imaging of sEVs and polystyrene beads.

Keywords:

Label-free detection Nanosphere Lithography (NSL)Nanosphere Settling Lithography (NSSL)Plasmonic Small Extracellular Vesicles (sEVs)

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Last Updated: May 19, 2026

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

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Published on: March 17, 2023

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Applied the platform for quantitative analysis of microRNA-21 expression in sEVs from patient plasma.

Main Results:

NSSL produced highly uniform, large-area AGNIS with low spatial variability across 60 mm × 24 mm substrates.
The NSSL-fabricated platform demonstrated high refractive-index sensitivity and exquisite detection limits for nanoparticles and sEVs.
Successfully enabled quantitative analysis of miR-21 in sEVs, identifying cancer-associated subpopulations.

Conclusions:

NSSL provides a scalable, economical, and uniform method for fabricating high-performance plasmonic biosensors.
The enhanced PANORAMA platform facilitates high-throughput, digital single-sEV analysis and molecular profiling.
This technology offers a promising pathway for translational liquid biopsy applications.