Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Isolation and Purification-Free Digital Single-Small Extracellular Vesicle Biosensing with Scalable Plasmonic Arrays.

bioRxiv : the preprint server for biology·2026

Same author

DHODH-Mediated Suppression of Ferroptosis Supports Radioresistance and Represents a Therapeutic Vulnerability in Lung Cancer.

Cancer research·2026

Same author

Initial Evaluation of Feasibility and Cutaneous Toxicity of Electron FLASH Radiotherapy Using a Standard-of-Care Fractionation Scheme in a Porcine Skin Model.

Cancers·2026

Same author

SHP2: A Redox-Sensitive Regulator Linking Immune Checkpoint Inhibitor Therapy to Cancer Treatment and Vascular Risk.

Antioxidants (Basel, Switzerland)·2025

Same author

SARS-CoV-2 mRNA vaccines sensitize tumours to immune checkpoint blockade.

Nature·2025

Same author

Causal AI-based clinical and radiomic analysis for optimizing patient selection in combined immunotherapy and SABR in early-stage NSCLC: a secondary analysis of the phase II I-SABR trial.

Journal for immunotherapy of cancer·2025

Search research articles

Related Experiment Video

Updated: Nov 30, 2025

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Plasmonic nano-aperture label-free imaging (PANORAMA).

Nareg Ohannesian¹, Ibrahim Misbah¹, Steven H Lin²

¹Department of Electrical and Computer Engineering, University of Houston, 4800 Calhoun Road, Houston, TX, 77204, USA.

Nature Communications

|November 17, 2020

Summary

We developed PANORAMA, a novel label-free optical imaging technique for detecting nanoscale objects. This method achieves diffraction-limited resolution and high surface sensitivity without lasers, enabling precise nanoparticle detection and sizing.

More Related Videos

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

Published on: August 29, 2025

Performing Spectroscopy on Plasmonic Nanoparticles with Transmission-Based Nomarski-Type Differential Interference Contrast Microscopy

Performing Spectroscopy on Plasmonic Nanoparticles with Transmission-Based Nomarski-Type Differential Interference Contrast Microscopy

Published on: June 5, 2019

Related Experiment Videos

Last Updated: Nov 30, 2025

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

Published on: August 29, 2025

Performing Spectroscopy on Plasmonic Nanoparticles with Transmission-Based Nomarski-Type Differential Interference Contrast Microscopy

Performing Spectroscopy on Plasmonic Nanoparticles with Transmission-Based Nomarski-Type Differential Interference Contrast Microscopy

Published on: June 5, 2019

Area of Science:

Nanotechnology
Optical Imaging
Plasmonics

Background:

Label-free optical imaging of nanoscale objects presents significant challenges.
Existing techniques like surface plasmon resonance (SPR) and localized surface plasmon resonance (LSPR) have limitations in resolution and sensitivity.
Current LSPR imaging methods often suffer from low light throughput and limited imaging capacity.

Purpose of the Study:

To introduce a new label-free optical imaging technique, PANORAMA (PlAsmonic NanO-apeRture lAbel-free iMAging).
To demonstrate PANORAMA's capability in detecting sub-100 nm dielectric nanoparticles with high resolution and sensitivity.
To showcase PANORAMA's potential for parallel detection, counting, sizing, and dynamic monitoring of nanoparticles.

Main Methods:

Utilizing ultra-near-field index modulation with plasmonic nano-apertures.
Employing unscattered light for detection, compatible with standard bright-field microscopy setups.
Implementing a system requiring only a lamp and camera, without lasers or interferometry.

Main Results:

Achieved diffraction-limited resolution in imaging nanoscale objects.
Demonstrated higher surface sensitivity compared to existing methods.
Successfully detected, counted, and sized individual dielectric nanoparticles down to 25 nm.
Enabled dynamic monitoring of nanoparticle distance to the plasmonic surface at millisecond timescales.

Conclusions:

PANORAMA offers a breakthrough in label-free nanoscale imaging, overcoming limitations of SPR and LSPR techniques.
The technique provides superior resolution, sensitivity, and imaging capacity using a simple, accessible setup.
PANORAMA facilitates parallel, high-throughput analysis and real-time monitoring of nanoparticles.