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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

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Related Experiment Video

Updated: May 8, 2025

Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid

Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid

Published on: December 4, 2017

Drug Repositioning Based on Cerebrospinal Fluid Proteomes Using Connectivity Map Framework.

Adriana Cortés¹, Silvia Romero-Murillo¹, Elena Anaya-Cubero¹

¹Clinical Neuroproteomics Unit, Proteomics Platform, Navarrabiomed, Hospital Universitario de Navarra (HUN), Universidad Pública de Navarra (UPNA), IdiSNA, Pamplona, Spain.

Methods in Molecular Biology (Clifton, N.J.)

|April 1, 2025

Summary

This summary is machine-generated.

Analyzing cerebrospinal fluid (CSF) proteomics can identify biomarkers for neurological disorders. This approach, combined with the Connectivity Map (CMap) framework, offers a cost-effective way to discover novel therapies for neurodegenerative diseases.

Keywords:

CSF Connectivity map Drug repurposing LC-MS/MS Label-Free Mass spectrometry Proteomics

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Last Updated: May 8, 2025

Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid

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Published on: December 4, 2017

A High Throughput, Multiplexed and Targeted Proteomic CSF Assay to Quantify Neurodegenerative Biomarkers and Apolipoprotein E Isoforms Status

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Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Published on: December 1, 2020

Area of Science:

Neuroscience
Biochemistry
Pharmacology

Background:

Cerebrospinal fluid (CSF) is proximate to the brain, making it a crucial source for neurological disorder biomarkers.
The inaccessibility of brain tissue necessitates alternative methods for biomarker discovery.
Altered CSF proteomes in neurological diseases offer opportunities for drug repurposing.

Purpose of the Study:

To explore the potential of CSF differential proteomics for identifying therapeutic strategies.
To leverage the Connectivity Map (CMap) framework for drug discovery in neurodegenerative diseases.

Main Methods:

Analysis of differential proteomics in CSF samples.
Utilizing the Connectivity Map (CMap) database to identify compounds and gene modifications.
Correlating proteomic signatures with CMap data to find potential therapeutic interventions.

Main Results:

Identification of specific proteomic alterations in CSF associated with neurological conditions.
Mapping these alterations to potential drug candidates and gene modulation strategies via CMap.
Demonstration of an efficient and cost-effective approach to therapeutic discovery.

Conclusions:

CSF differential proteomics is a viable strategy for biomarker discovery in neurological disorders.
The CMap framework effectively aids in identifying potential therapeutic agents for neurodegenerative diseases.
This integrated approach offers a promising pathway for developing novel treatments.