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Selection of MDM2-binding peptides in Escherichia coli using an engineered split intein and aminoglycoside
Yutaro Watanabe1, Chiori Tezuka2, Kana Nobusawa1
1Graduate School of Science and Technology, Gunma University, 1-5-1 Tenjin-cho, Kiryu, Gunma 376-8515, Japan. ttakahas@gunma-u.ac.jp.
Chemical Communications (Cambridge, England)
|April 1, 2025
Abstract:
A protein trans-splicing (PTS)-based method for screening the peptides capable of binding to a target protein was developed using an engineered split intein and an aminoglycoside phosphotransferase. Two peptides selected against MDM2 bind to MDM2 with high affinity and a peptide can induce apoptosis in SJSA-1 cells.
