Challenges and opportunities in characterisation of phytochemicals in pasture-fed meat produce

Consumers are becoming increasingly concerned with the origin, welfare, and nutritional quality of meat they consume. Existing literature suggests that pasture-fed livestock can absorb phytonutrients from their feed into their meat. Advanced analytical methods such as liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS) have now made it possible to identify plant-derived non-volatile compounds in meat. These biomarkers are crucial for authenticating pasture-fed meat to underpin stringent meat quality regulations and assurance systems, thus enhancing industry reputation and competitiveness. Studies have shown that animals fed with a diet rich in phytochemicals exhibit higher total polyphenol content (TPC) in their muscle compared to those fed with a concentrate diet. However, there is limited information available on the bioavailability of phytochemicals in meat. The aim of this review is to comprehensively analyse the latest methods for extracting, purifying, and characterising non-volatile biomarkers in meat, and to evaluate their effectiveness as indicators of meat authenticity and nutrition.